Induction of lipid droplets in non-macrophage cells as well as macrophages by liposomes and exosomes
Induction of lipid droplets in non-macrophage cells as well as macrophages by liposomes and exosomes
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DOI：
10.1016/j.bbrc.2019.01.078
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发表时间：
2019-02-26
影响因子：
3.1
通讯作者：
Hinuma, Shuji
中科院分区：
文献类型：
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作者：
Fujita, Kazuyo;Somiya, Masaharu;Hinuma, Shuji
已有报道，磷脂纳米颗粒（PNP）包括脂质体和外泌体可以有效地诱导巨噬细胞中的脂滴（LD）。然而，在非巨噬细胞中，PNP对LD诱导的影响尚未得到彻底研究。在这份报告中，我们直接比较了非巨噬细胞和巨噬细胞细胞系的LD诱导的各种制剂的脂质体含有磷脂酰丝氨酸和外来体。本研究中测试的所有非巨噬细胞细胞系以及巨噬细胞系显示出对这些PNP的明显LD诱导，尽管LD诱导在非巨噬细胞系中的功效变化很大。我们的研究结果表明，LD的形成是一个共同的和至关重要的反应，PNPs在哺乳动物细胞不仅在巨噬细胞，而且在非巨噬细胞。(C)2019爱思唯尔公司All rights reserved.
It has been reported that phospholipid nanoparticles (PNPs) including liposomes and exosomes could efficiently induce lipid droplets (LDs) in macrophages. However, in non-macrophage cells, the effects of PNPs on the induction of LDs have not been thoroughly investigated. In this report, we directly compared non-macrophage and macrophage cell lines in terms of LD induction by various formulation of liposomes containing phosphatidylserine and exosomes. All non-macrophage cell lines as well as macrophage cell lines tested in this study showed evident LD induction in response to these PNPs, though the efficacy of LD induction in non-macrophage cell lines varied considerably. Our results suggest that LD formation is a common and crucial response to PNPs in mammalian cells not only in macrophages but also in non-macrophage cells. (C) 2019 Elsevier Inc. All rights reserved.