Pinpoint injection of microtools for minimally invasive micromanipulation of microbe by laser trap
Pinpoint injection of microtools for minimally invasive micromanipulation of microbe by laser trap
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DOI：
10.1109/tmech.2003.809129
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发表时间：
2003-03
影响因子：
6.4
通讯作者：
F. Arai;H. Maruyama;T. Sakami;A. Ichikawa;T. Fukuda
中科院分区：
文献类型：
--
作者：
F. Arai;H. Maruyama;T. Sakami;A. Ichikawa;T. Fukuda
本文报道了利用激光捕获微型工具，以最小的激光照射到靶上来传输目标微生物。微型工具(MT)的尺寸约为微米。利用显微镜下的聚焦激光操控微生物培养物来操控目标微生物。在此，我们提出了一种在充满液体的微腔中的所需位置精确注射MTS的方法。首先，我们将MTS的注射方法分为四类。在这里，我们采用了一种新的方法将MTS安装在微腔内。我们开发了一款MT保持芯片来安装MTS。将MTS注入微室，并通过激光扫描微操作器成功地操纵MTS以输送目标微生物进行分离。该方法可用于MTS的针尖注射和间接微操作分离。
This paper reports transportation of the target microbe by the laser trapped microtools with minimum laser irradiation to the target. The size of a microtool (MT) is around micrometer. The MTs are manipulated by the focused laser under the microscope to manipulate the target microbe. Here we propose a pinpoint injection method of MTs at the desired location in the microchamber, which is filled with liquid. At first, we classified the injection method of the MTs in four categories. Here we employed a new method to install the MTs inside the microchamber. We developed a MT holding chip to install the MTs. The MTs were injected in the microchamber, and were manipulated successfully by the laser scanning micromanipulator to transport the target microbe for separation. The proposed method is useful for the pinpoint injection of MTs and separation by the indirect micromanipulation.