Nutrient homeostasis, metabolism of reserves, and seedling vigor as affected by seed priming in coarse rice
Nutrient homeostasis, metabolism of reserves, and seedling vigor as affected by seed priming in coarse rice
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DOI：
10.1139/b06-088
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发表时间：
2006-08-01
期刊：
影响因子：
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通讯作者：
Mahmood, T.
中科院分区：
文献类型：
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作者：
Farooq, M.;Basra, S. M. A.;Mahmood, T.
研究了引发对水稻种子萌发、幼苗活力、离子稳态和淀粉代谢的影响。引发处理包括预发芽（传统的水稻育苗方法）、水引发48 h、KCl或CaCl_2（psi s-1.25MPa溶液）浸种硬化24 h、抗坏血酸引发（10 mg中心点L ~（-1））48 h和硬化24 h。与对照相比，所有引发处理（除预萌发外）均缩短了种子萌发时间，提高了萌发率和同步化率，提高了芽长和根长、幼苗鲜质量和干质量、次生根数、还原糖浓度和α-淀粉酶活性，但不同处理的变化程度不同。这些种子处理导致较高的发芽率，这可能是由于克服休眠。对于这些参数，KCl的渗透硬化比CaCl 2更有效。氮浓度保持不受影响的幼苗，但是，在种子和幼苗中的Ca 2+浓度更大的种子与CaCl 2 hardenerd比所有其他处理，包括控制。种子引发提高了种子和幼苗中的K+浓度，从而提高了α-淀粉酶活性。种子K+浓度与淀粉酶活性、还原糖浓度与淀粉酶活性、幼苗干重、次生根数均呈正相关。渗透硬化与氯化钾表现优于所有其他处理，包括对照。引发改善了激活α-淀粉酶的K+平衡，这是种子活力的基础。
The influence of seed priming on germination, seedling vigor, ion homeostasis, and starch metabolism in coarse rice was studied. Priming treatments included pregermination (a traditional soaking method being used for rice nursery preparation), hydropriming for 48h, osmohardening with KCl or CaCl2 (psi s -1.25 MPa solution) for 24h, ascorbate priming (10 mg center dot L-1) for 48h, and hardening for 24h. Compared with controls, all priming treatments (except pregermination) reduced the time to start germination, improved the rate of germination and synchronization, and the length of shoot and root, seedling fresh and dry mass, number of secondary roots, the concentration of reducing sugars, and alpha-amylase activity, although the extent of these changes was different in seeds subjected to different treatments. These seed treatments resulted in higher germination that might be due to overcoming dormancy. Osmohardening with KCl was more effective than CaCl2 for these parameters. Nitrogen concentration remained unaffected in seedlings; however, Ca2+ concentrations in both seeds and seedlings were greater in seeds osmohardenerd with CaCl2 than with all other treatments, including the control. Seed priming enhanced K+ concentration in both seeds and seedlings, leading to improved alpha-amylase activity. There were positive correlations between seed K+ concentration and amylase activity, and the concentration of reducing sugars with amylase activity, seedling dry mass, or number of secondary roots. Osmohardening with KCl performed better than all other treatments including control. Priming improved the K+ balance that activates alpha-amylase, a basis for seed invigoration.