Sol-gel synthesis and electrospraying of biodegradable (P2O5)55-(CaO)30-(Na2O)15 glass nanospheres as a transient contrast agent for ultrasound stem cell imaging.
Sol-gel synthesis and electrospraying of biodegradable (P2O5)55-(CaO)30-(Na2O)15 glass nanospheres as a transient contrast agent for ultrasound stem cell imaging.
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DOI：
10.1021/nn506789y
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发表时间：
2015-02-24
期刊：
影响因子：
17.1
通讯作者：
Knowles, Jonathan C.
中科院分区：
文献类型：
--
作者：
Foroutan, Farzad;Jokerst, Jesse V.;Gambhir, Sanjiv S.;Vermesh, Ophir;Kim, Hae-Won;Knowles, Jonathan C.
超声成像具有毫秒级的时间分辨率和亚毫米级的空间分辨率，是医学领域的有力工具。然而，目前这一代声学造影剂主要限于血管靶，因为它们的尺寸很大。纳米颗粒具有作为超声分子成像造影剂的潜力。基于二氧化硅的纳米颗粒在这里显示出了希望，但是它们的缓慢降解速率可能限制它们作为造影剂的应用。磷酸盐玻璃是一种有吸引力的替代品，具有可控的降解速率和易于在体内代谢的降解组分。在这项研究中，可生物降解的P2 O 5-CaO-Na 2 O磷酸盐基玻璃纳米球（PGNs）的合成和其特征在于作为超声成像造影剂。使用扫描电子显微镜（SEM）、能量色散X射线光谱（EDX）、X射线衍射（XRD）、31 P核磁共振（31 P MAS-NMR）和傅里叶变换红外（FTIR）光谱表征PGN的结构。SEM图像显示直径尺寸范围为200-500 nm的球形。XRD、31 P NMR和FTIR结果显示PGNs主要由Q1和Q2磷酸单元组成的无定形和玻璃态性质。我们使用这种对比标记间充质干细胞，并分别确定体外和体内检测限为5 μg/mL和9 μg/mL。可以用超声成像测量低至4000的细胞计数，在成像所需的剂量下没有细胞毒性。重要的是，离子释放研究证实这些PGN生物降解到水性介质中，降解产物可以在体内容易代谢。
Ultrasound imaging is a powerful tool in medicine because of the millisecond temporal resolution and sub-millimeter spatial resolution of acoustic imaging. However, the current generation of acoustic contrast agents is primarily limited to vascular targets due to their large size. Nano-size particles have the potential to be used as a contrast agent for ultrasound molecular imaging. Silica-based nanoparticles have shown promise here, however their slow degradation rate may limit their applications as a contrast agent. Phosphate-based glasses are an attractive alternative with controllable degradation rate and easily metabolized degradation components in the body. In this study, biodegradable P2O5-CaO-Na2O phosphate-based glass nanospheres (PGNs) were synthesized and characterized as contrast agents for ultrasound imaging. The structure of the PGNs was characterised using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), 31P nuclear magnetic resonance (31P MAS-NMR), and Fourier transform infrared (FTIR) spectroscopy. The SEM images indicated a spherical shape with a diameter size range of 200-500 nm. The XRD, 31P NMR and FTIR results revealed the amorphous and glassy nature of PGNs that consisted of mainly Q1 and Q2 phosphate units. We used this contrast to label mesenchymal stem cells and determined in vitro and in vivo detection limits of 5 and 9 μg/mL, respectively. Cell counts down to 4000 could be measured with ultrasound imaging with no cytoxicity at doses needed for imaging. Importantly, ion release studies confirmed these PGNs biodegrade into aqueous media with degradation products that can be easily metabolized in the body.
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影响因子：
168.9
作者：
Makkar, Raj R.;Smith, Rachel R.;Cheng, Ke;Malliaras, Konstantinos;Thomson, Louise E. J.;Berman, Daniel;Czer, Lawrence S. C.;Marban, Linda;Mendizabal, Adam;Johnston, Peter V.;Russell, Stuart D.;Schuleri, Karl H.;Lardo, Albert C.;Gerstenblith, Gary;Marban, Eduardo
通讯作者：
Marban, Eduardo
影响因子：
6.7
作者：
Casciaro, Sergio;Conversano, Francesco;Gigli, Giuseppe
通讯作者：
Gigli, Giuseppe
影响因子：
3.5
作者：
BYUN, JO;KIM, BH;IZYNEEV, AA
通讯作者：
IZYNEEV, AA
介孔二氧化硅纳米粒子的体内生物分布和尿液排泄：粒径和聚乙二醇化的影响
影响因子：
13.3
作者：
He, Qianjun;Zhang, Zhiwen;Shi, Jianlin
通讯作者：
Shi, Jianlin
DOI：
10.1001/jama.2012.25321
发表时间：
2012-12-12
期刊：
JAMA
影响因子：
--
作者：
Hare JM;Fishman JE;Gerstenblith G;DiFede Velazquez DL;Zambrano JP;Suncion VY;Tracy M;Ghersin E;Johnston PV;Brinker JA;Breton E;Davis-Sproul J;Schulman IH;Byrnes J;Mendizabal AM;Lowery MH;Rouy D;Altman P;Wong Po Foo C;Ruiz P;Amador A;Da Silva J;McNiece IK;Heldman AW;George R;Lardo A
通讯作者：
Lardo A