Spatial differences in molecular characteristics of the pontine parabrachial nucleus
Spatial differences in molecular characteristics of the pontine parabrachial nucleus
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DOI：
10.1016/j.brainres.2009.07.098
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发表时间：
2009-10-16
期刊：
影响因子：
2.9
通讯作者：
Abe, Keiko
中科院分区：
文献类型：
--
作者：
Maeda, Naohiro;Onimura, Mayuko;Abe, Keiko
脑桥臂旁核（PBN）中的神经元为一般内脏感觉、躯体感觉、味觉和自主神经系统提供信号，并且执行这些功能的各种PBN神经元是混合的。在这项研究中，我们分析了PBN的基因表达谱在雄性Wistar大鼠和PBN和三叉神经的主要感觉核（Pr 5）的基因表达的数据。使用这些数据结合原位杂交分析，我们确定的基因，表现出较高的表达在PBN比在Pr 5。我们的研究结果表明，在PBN的表达模式是不同的基因：Fxyd 6，syt 5，和plxnc 1在PBN的许多神经元群体中表达，而钙和asb 4的表达模式仅限于中央外侧亚核和腰部区域。此外，calcr和asb 4的表达模式是不同的神经递质/神经肽，如神经降压素和降钙素基因相关肽。Satb 2在腰部区域特异性表达，这是味觉所必需的。对PBN中空间分布的深入分析使得能够将基因分类为七种特征性空间表达模式。表达签名显着不同的喙半，mediodorsal一半，腹外侧三分之一的PBN的亚核，表明亚核的空间排列和相应的神经元的分子特征之间的相关性。因此，我们的研究结果提供了有价值的信息，PBN神经元的分子特征和神经传递机制，传递特定类型的信息。(C)2009爱思唯尔有限公司版权所有。
Neurons in the Pontine parabrachial nucleus (PBN) transduce signals for the general visceral sensory, somatic sensory, gustatory, and autonomic nervous systems, and the various PBN neurons that perform these functions are intermingled. In this study, we analyzed PBN gene expression profiles in male Wistar rats and obtained data on gene expression in the PBN and the principal sensory nucleus of the trigeminal nerve (Pr5). Using these data in combination with in situ hybridization analyses, we identified genes that showed higher expression in the PBN than in Pr5. Our findings indicate that expression patterns in the PBN were different for different genes: Fxyd6, syt5, and plxnc1 were expressed in many neuron populations in the PBN, while the expression patterns of calcr and asb4 were restricted to the central lateral subnucleus and waist area. Furthermore, calcr and asb4 expression patterns were distinct from those of neurotransmitters/neuropeptides such as neurotensin and calcitonin gene-related peptides. Satb2 was specifically expressed in the waist area, which is essential for gustation. in-depth analysis of spatial distribution in the PBN enabled classification of the genes into seven characteristic spatial expression patterns. Expression signatures differed significantly in the subnuclei of the rostral half, mediodorsal half, and ventrolateral third of the PBN, indicating a correlation between the spatial arrangement of the subnuclei and the molecular characteristics of the corresponding neurons. Thus, our results provide valuable information regarding the molecular features and neurotransmission mechanisms of PBN neurons that transmit specific types of information. (C) 2009 Elsevier B.V. All rights reserved.