Expression of Rac3 in human brain tumors
Expression of Rac3 in human brain tumors
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DOI：
10.1016/j.jocn.2004.08.013
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发表时间：
2005-06-01
影响因子：
2
通讯作者：
Hong, YR
中科院分区：
文献类型：
--
作者：
Hwang, SL;Chang, JH;Hong, YR
Rac3可能在肿瘤生长中起重要作用，但对其在人类肿瘤组织中的表达和突变知之甚少。采用逆转录聚合酶链式反应(RT-PCR)和DNA测序的方法检测了细胞中rac3基因的表达情况。19%(5/26)的脑肿瘤、3/9(33%)的脑膜瘤、1/11(9%)的星形细胞瘤和1/6(17%)的垂体腺瘤出现了rac3基因的过表达。在3例rac3过表达的脑膜瘤中，2例为复发性脑膜瘤。星形细胞瘤中只有多形性胶质母细胞瘤有rac3的高表达。在63%(12/19)的脑肿瘤、57.1%(4/7)的脑膜瘤、80%(4/5)的垂体腺瘤和57.1%(4/7)的星形细胞瘤中发生了rac3基因突变。除1例星形细胞瘤外，其他4例肿瘤(3例脑膜瘤和1例垂体腺瘤)均未检出rac3基因突变。我们的数据是第一个关于人脑肿瘤中rac3过表达和突变的频率的报告。过度表达可能与肿瘤的侵袭性行为有关。Rac3基因表达与突变之间的关系有待进一步研究。(C)2005爱思唯尔有限公司。保留所有权利。
Rac3 may play an important role in tumor growth but little is known about its expression and mutation in human tumor tissues. We examined the expression of Rac3 using RT-PCR and mutation of the Rac3 gene by DNA sequencing. Overexpression of the Rac3 gene occurred in 19% (5/26) of brain tumors; 3 of 9 (33%) meningiomas, 1 of 11 (9%) astrocytomas and 1 of 6 (17%) pituitary adenomas. Two of the 3 meningiomas with Rac3 overexpression were recurrent meningionnas. The only astrocytoma with Rac3 overexpression was a glioblastoma multiforme. Mutation of the Rac3 gene occurred in 63% (12/19) of brain tumours; 4 of 7 (57.1 %) meningiomas, 4 of 5 (80%) pituitary adenomas and 4 of 7 (57.1 %) astrocytomas. Except in one astrocytoma, the other four tumors with Rac3 overexpression (3 meningiomas and one pituitary adenoma) did not have Rac3 mutations. Our data is the first report of the frequency of Rac3 overexpression and mutation in human brain tumors. Overexpression may be associated with aggressive tumor behavior. The relationship between Rac3 expression and mutation requires further investigation. (c) 2005 Elsevier Ltd. All rights reserved.