Ca2+-ATPase inhibitors and Ca2+-ionophore induce osteoclast-like cell formation in the cocultures of mouse bone marrow cells and calvarial cells.
Ca2+-ATPase inhibitors and Ca2+-ionophore induce osteoclast-like cell formation in the cocultures of mouse bone marrow cells and calvarial cells.
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Ca2-ATP酶抑制剂和Ca2-离子载体在小鼠骨髓细胞和颅骨细胞的共培养物中诱导破骨细胞样细胞形成。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
K. Nagai
中科院分区：
文献类型：
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作者：
M. Takami;J. Woo;N. Takahashi;T. Suda;K. Nagai
源自造血细胞的破骨细胞是负责骨吸收的多核细胞。我们发现环吡唑酸（CPA）、塔素素（TG）和2,5-二-（t-丁基）-1,4-对苯二酚（BHQ）在小鼠颅骨来源的基质细胞和造血细胞（如骨髓细胞和脾脏细胞）共培养中诱导破骨细胞样细胞（OCL）的形成。这些化合物诱导的破骨细胞表现出抗酒石酸酸性磷酸酶活性和窝形成活性等典型破骨细胞特征。这些化合物被称为内质网(ER)/肌浆网（SR） Ca2+- atp酶抑制剂，通过抑制位于ER/SR膜上的Ca2+- atp酶活性来增加细胞内Ca2+水平。Ca2+离子载体，如离子霉素，增加细胞内Ca2+水平也刺激OCL的形成在共培养。由这些化合物诱导的造血细胞向ocl分化需要颅骨细胞的存在。这些结果表明，细胞内Ca2+水平的增加可能是在颅骨细胞存在时诱导破骨细胞分化的信号通路的一部分。
Osteoclasts which derive from hemopoietic cells are multinucleated cells responsible for bone resorption. We found that cyclopiazonic acid (CPA), thapsigargin (TG), and 2,5-di-(t-butyl)-1,4-hydroquinone (BHQ) induced osteoclast-like cell (OCL) formation in cocultures of mouse calvaria-derived stromal cells and hemopoietic cells such as bone marrow cells and spleen cells. OCLs induced by these compounds showed typical characteristics of osteoclasts such as tartrate-resistant acid phosphatase activity and pit forming activity. These compounds are known as endoplasmic reticulum (ER)/sarcoplasmic reticulum (SR) Ca2+-ATPase inhibitors that increase intracellular Ca2+ levels by inhibiting Ca2+-ATPase activity located in the membrane of ER/SR. Ca2+-ionophores such as ionomycin which increase intracellular Ca2+ levels also stimulated OCL formation in the cocultures. Differentiation of hemopoietic cells into OCLs induced by these compounds required the presence of calvarial cells. These results indicate that an increase of intracellular Ca2+ levels may be a part of signaling pathways to induce osteoclast differentiation in the presence of calvarial cells.
毒胡萝卜素和环吡嗪酸引发小鼠腹膜巨噬细胞中 IL-6 mRNA 表达和 IL-6 分泌的快速且显着的增加。
DOI：
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发表时间：
1995
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Bost,KL;Mason,MJ
通讯作者：
Mason,MJ
毒胡萝卜素持续消耗细胞内钙池及其对细胞生长的影响。
DOI：
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发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Ghosh,TK;Bian,JH;Short,AD;Rybak,SL;Gill,DL
通讯作者：
Gill,DL
影响因子：
4
作者：
PUTNEY, JW
通讯作者：
PUTNEY, JW