FRIZZY GENES OF MYXOCOCCUS-XANTHUS ARE INVOLVED IN CONTROL OF FREQUENCY OF REVERSAL OF GLIDING MOTILITY
FRIZZY GENES OF MYXOCOCCUS-XANTHUS ARE INVOLVED IN CONTROL OF FREQUENCY OF REVERSAL OF GLIDING MOTILITY
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DOI：
10.1073/pnas.82.24.8767
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发表时间：
1985-12-01
影响因子：
11.1
通讯作者：
ZUSMAN, DR
中科院分区：
文献类型：
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作者：
BLACKHART, BD;ZUSMAN, DR
黄色粘球菌是一种革兰氏阴性细菌，具有复杂的生命周期，包括子实体的形成。卷曲（frz）突变体无法正常聚集，而是形成模糊的丝状聚集体。我们发现这些突变体在滑翔运动过程中控制细胞逆转方面存在缺陷。野生型细胞大约每 6.8 分钟改变一次滑动方向；由于反转之间的内部差异可能很大，因此会出现净变动。 frzA-C、-E 和 -F 突变体很少改变其运动方向，大约每 2 小时一次。这些突变体不能正常聚集并在结果琼脂或运动琼脂上产生卷曲的丝状菌落。相比之下，frzD 突变体非常频繁地反转其运动方向，大约每 2.2 分钟一次；单个细胞几乎没有表现出净运动，并形成边缘光滑的“不运动”型集落。 frzD 基因座的遗传分析表明，该基因座的突变对于野生型等位基因而言可能是显性的，并且其基因产物必须与其他 frz 基因产物相互作用。我们的结果表明 frz 基因是负责该生物体定向运动的系统的一部分。
Myxococcus xanthus, a Gram-negative bacterium, has a complex life cycle that includes fruiting body formation. Frizzy (frz) mutants are unable to aggregate normally, instead forming fuzzy filamentous aggregates. We have found that these mutants are defective in the control of cell reversal during gliding motility. Wild-type cells reverse their direction of gliding about every 6.8 min; net movement occurs since the internal between reversals can vary widely. The frzA-C, -E and -F mutants reverse their direction of movement very rarely, about once every 2 hr. These mutants cannot aggregate normally and give rise to frizzy filamentous colonies on fruiting agar or motility agar. In contrast, frzD mutants reverse their direction of movement very frequently, about once every 2.2 min; individual cells show little net movement and form smooth-edged "nonmotile" type colonies. Genetic analysis of the frzD locus shows that mutations in this locus can be dominant to the wild-type allele and that its gene product(s) must interact with the other frz gene products. Our results suggest that frz genes are part of a system responsible for directed movement of this organism.