Gut microbiota composition influences outcomes of skeletal muscle nutritional intervention via blended protein supplementation in posttransplant patients with hematological malignancies
Gut microbiota composition influences outcomes of skeletal muscle nutritional intervention via blended protein supplementation in posttransplant patients with hematological malignancies
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肠道微生物群组成影响血液恶性肿瘤移植后患者通过补充混合蛋白质进行骨骼肌营养干预的结果
DOI：
10.1016/j.clnu.2020.04.030
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发表时间：
2021-01-01
影响因子：
6.3
通讯作者：
Wang, Jiaqi
中科院分区：
文献类型：
--
作者：
Ren, Guangxu;Zhang, Jianping;Wang, Jiaqi
背景：骨骼肌萎缩是造血干细胞移植(HSCT)后一个重要且独立的生存预测因子。我们先前的研究发现，大豆-乳清混合蛋白(SWP)可以改善急性白血病患者的肌肉质量。目的：探讨影响营养干预后肌肉预后的潜在因素。方法：本病例对照研究包括13例接受HSCT但半年内肌肉功能改善不佳的患者。在SWP干预2个月后，将受试者分为两组(MSI：肌肉状态改善；MNI：肌肉状态未改善)。用16S rDNA测序、主坐标分析(PCoA)和PICRUST算法分析两组间肠道微生物区系的组成、结构和功能。结果：SWP显著改善肌肉状况(肌肉面积由330.4 mm(2)增加到384.8 mm(2)，p=0.02；肌力：从19.2 kg增加到21.3 kg，p=0.04)。然而，有一小部分受试者的肌肉状况没有得到有效的改善。经SWP干预后，MSI组肠道微生物区系多样性(Shannon：1.7~3.8，p=0.0 1；Simpson：0.6~0.8，p=0.015)显著增加，而MNI组无明显变化。主成分分析(PCA)揭示了MSI组中基线-MSI和终点-MSI时间点的微生物区系的单独分组。微生物丰度变化在MSI组(75%的变化属增加)和MNI组(80%的变化属减少)之间发现相反的模式。三种细菌分类群(负相关：链球菌；正相关：反式鲁米诺球菌和韦龙氏杆菌)与肌肉改善效果显著相关。通过PICRUST算法预测，与肌肉代谢相关的磷酸戊糖(p=0.048)和氨基酸生物合成(p=0.039)在微卫星组均发生了显著变化。结论：肠道微生物区系在肌肉代谢调节中起着重要作用。(C)2020爱思唯尔有限公司和欧洲临床营养和代谢学会。版权所有。
Background: Skeletal muscle atrophy is an important and independent predictor of survival after hematopoietic stem cell transplantation (HSCT). Our previous study found that soy-whey blended protein (SWP) can improve muscle mass in acute leukemia patients.Objective: We aimed to explore potential factors that influence muscle outcomes after nutritional intervention.Methods: In this case-control study, 13 patients who received HSCT and failed to improve muscle function within half a year were included. After two months of SWP intervention, the subjects were divided into two groups (MSI: muscle status improved; MNI: muscle status not improved). 16S rDNA sequencing, principal coordinate analysis (PCoA) and the PICRUSt algorithm were used to analyze the composition, structure and function of the intestinal microbiota between the groups. This study was registered in the Chinese Clinical Trial Registry (ChiCTR 1800017765).Results: SWP significantly improved muscle status (muscle area: from 330.4 mm(2) to 384.8 mm(2), p = 0.02; muscle strength: from 19.2 kg to 21.3 kg, p = 0.04). However, there were a small number of subjects whose muscle status was not effectively improved. After SWP intervention, the diversity (Shannon: from 1.7 to 3.8, p = 0.01; Simpson: from 0.6 to 0.8, p = 0.015) of the intestinal microbiota in the MSI group increased significantly, whereas that in the MNI group did not. Principal component analysis (PCA) revealed separate groupings of the microbiota of the Baseline-MSI and Endpoint-MSI time points in the MSI group. Opposite patterns of microbial abundance change were found between the MSI group (75% of changed genera were increased) and the MNI group (80% of changed genera were decreased). Three bacterial taxa (negative correlation: Streptococcus; positive correlations: Ruminococcus and Veillonella) were significantly related to muscle improvement outcomes. Both pentose phosphate (p = 0.048) and amino acid biosynthesis (p = 0.039), which are related to muscle metabolism, were found to be significantly changed in the MSI group through PICRUSt algorithm prediction.Conclusions: Our results suggest that the intestinal microbiota plays important roles in the regulation of muscle metabolism. (C) 2020 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.