Simple Surface Modification of Poly(dimethylsiloxane) via Surface Segregating Smart Polymers for Biomicrofluidics
Simple Surface Modification of Poly(dimethylsiloxane) via Surface Segregating Smart Polymers for Biomicrofluidics
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DOI：
10.1038/s41598-019-43625-5
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发表时间：
2019-05-14
影响因子：
4.6
通讯作者：
Asatekin, Ayse
中科院分区：
文献类型：
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作者：
Gokaltun, Aslihan;Kang, Young Bok (Abraham);Asatekin, Ayse
聚二甲基硅氧烷（PDMS）可能是芯片实验室和其他生物医学应用中最受欢迎的微流体设备材料。然而，PDMS的疏水性导致蛋白质和其他分子如治疗药物的非特异性吸附，限制了其更广泛的应用。在这里，我们介绍了一种简单的方法来制备PDMS材料，以提高亲水性，减少非特异性蛋白质吸附，同时保持细胞的生物相容性，透明度和良好的机械性能，而不需要任何后固化表面处理。该方法利用由聚（乙二醇）（PEG）和PDMS链段（PDMS-PEG）组成的智能共聚物，当在器械制造过程中与PDMS混合时，该共聚物自发分离至与水溶液接触的表面并降低疏水性，而无需任何额外的制造步骤。PDMS-PEG改性的PDMS样品显示出低至23.6度+/-1度的接触角，并保持这种亲水性至少20个月。使用毛细管流动实验证实了它们改善的润湿性。改良后的器械对白蛋白、溶菌酶和免疫球蛋白G的非特异性吸附显著降低。修饰的PDMS是生物相容的，当用于使用原代大鼠肝细胞的简单的肝芯片模型时，没有显示出不良反应。这种PDMS修饰方法可进一步应用于分析分离、生物传感、细胞研究和药物相关研究。
Poly(dimethylsiloxane) (PDMS) is likely the most popular material for microfluidic devices in lab-on-a-chip and other biomedical applications. However, the hydrophobicity of PDMS leads to non-specific adsorption of proteins and other molecules such as therapeutic drugs, limiting its broader use. Here, we introduce a simple method for preparing PDMS materials to improve hydrophilicity and decrease non-specific protein adsorption while retaining cellular biocompatibility, transparency, and good mechanical properties without the need for any post-cure surface treatment. This approach utilizes smart copolymers comprised of poly(ethylene glycol) (PEG) and PDMS segments (PDMS-PEG) that, when blended with PDMS during device manufacture, spontaneously segregate to surfaces in contact with aqueous solutions and reduce the hydrophobicity without any added manufacturing steps. PDMS-PEG-modified PDMS samples showed contact angles as low as 23.6 degrees +/- 1 degrees and retained this hydrophilicity for at least twenty months. Their improved wettability was confirmed using capillary flow experiments. Modified devices exhibited considerably reduced non-specific adsorption of albumin, lysozyme, and immunoglobulin G. The modified PDMS was biocompatible, displaying no adverse effects when used in a simple liver-on-a-chip model using primary rat hepatocytes. This PDMS modification method can be further applied in analytical separations, biosensing, cell studies, and drug-related studies.