Cancer cell invasion and EMT marker expression: a three-dimensional study of the human cancer-host interface
Cancer cell invasion and EMT marker expression: a three-dimensional study of the human cancer-host interface
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DOI：
10.1002/path.4416
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发表时间：
2014-11-01
影响因子：
7.3
通讯作者：
Wellner, U. F.
中科院分区：
文献类型：
--
作者：
Bronsert, P.;Enderle-Ammour, K.;Wellner, U. F.
癌细胞侵袭发生在癌-宿主界面，是远处转移的先决条件。目前的生物学和临床概念之间的关系，如细胞迁移模式，肿瘤出芽和上皮间质转化（EMT）仍然不清楚在几个方面，特别是对于“真实的”的情况下，在人类癌症。我们开发了一种新的方法，提供了精确的三维（3D）信息的微观形态和基因表达，在几乎无限的空间范围内，从连续免疫染色组织切片重建。对人类胰腺癌、结直肠癌、肺癌和乳腺癌中肿瘤-宿主界面处肿瘤出芽的定量3D评估表明，集体细胞迁移是癌细胞侵袭的机制，而单个癌细胞迁移似乎几乎不存在。出芽的肿瘤细胞显示出向纺锤形以及圆形形态的转变。这与E-钙粘蛋白染色强度降低和从膜染色向胞质染色的转变以及核ZEB 1表达增加有关。版权所有（c）2014大不列颠和爱尔兰病理学会。由John Wiley & Sons有限公司出版
Cancer cell invasion takes place at the cancer-host interface and is a prerequisite for distant metastasis. The relationships between current biological and clinical concepts such as cell migration modes, tumour budding and epithelial-mesenchymal transition (EMT) remains unclear in several aspects, especially for the 'real' situation in human cancer. We developed a novel method that provides exact three-dimensional (3D) information on both microscopic morphology and gene expression, over a virtually unlimited spatial range, by reconstruction from serial immunostained tissue slices. Quantitative 3D assessment of tumour budding at the cancer-host interface in human pancreatic, colorectal, lung and breast adenocarcinoma suggests collective cell migration as the mechanism of cancer cell invasion, while single cancer cell migration seems to be virtually absent. Budding tumour cells display a shift towards spindle-like as well as a rounded morphology. This is associated with decreased E-cadherin staining intensity and a shift from membranous to cytoplasmic staining, as well as increased nuclear ZEB1 expression. Copyright (c) 2014 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.