ISOLATION OF UV-INDUCIBLE TRANSCRIPTS FROM SCHIZOSACCHAROMYCES-POMBE
ISOLATION OF UV-INDUCIBLE TRANSCRIPTS FROM SCHIZOSACCHAROMYCES-POMBE
复制标题
DOI：
10.1006/bbrc.1994.2043
复制
发表时间：
1994-07-29
影响因子：
3.1
通讯作者：
PARK, SD
中科院分区：
文献类型：
--
作者：
LEE, JK;PARK, EJ;PARK, SD
利用差减杂交技术从粟酒裂殖酵母中分离到4个紫外线诱导的cDNA克隆，即UV115、UV118、UV122和UV131。这些克隆的所有转录物在UV照射后1小时内被快速诱导约5至10倍，并且这些克隆的核苷酸序列与数据库中的已知基因没有显示出任何显著的序列同源性。UV118和UV131的转录仅受紫外线照射诱导，UV122的转录也受烷化剂诱导，表明这些转录的诱导是对DNA损伤的特异性反应。然而，UV115的转录水平也增加了其他细胞毒性剂，包括热休克。这些结果表明，UV115可能是一个胁迫响应基因。(C)1994年出版社出版。
Four UV-inducible cDNA clones, UV115, UV118, UV122 and UV131, were isolated from Schizosaccharomyces pombe by subtraction hybridization. All transcripts of these clones were rapidly induced about 5 to 10 fold within 1 hour after UV-irradiation and the nucleotide sequences of these clones did not showed any significant sequence homology to the known genes in the data bases. Transcripts of UV118 and UV131 were induced only by UV-irradiation and those of UV122 were also induced by alkylating agents, suggesting that inductions of these transcripts are specific responses to DNA damages. However, transcript levels of UV115 were also increased by other cytotoxic agents including heat shock. These results indicate that UV115 might be a stress responsive gene. (C) 1994 Academic Press, Inc.