Partially replacing cornstarch in a high-concentrate diet with sucrose inhibited the ruminal trans-10 biohydrogenation pathway in vitro by changing populations of specific bacteria.
Partially replacing cornstarch in a high-concentrate diet with sucrose inhibited the ruminal trans-10 biohydrogenation pathway in vitro by changing populations of specific bacteria.
复制标题
用蔗糖部分替代高浓度饮食中的玉米淀粉，通过改变特定细菌的种群，在体外抑制瘤胃反式10生物氢化途径
DOI：
10.1186/s40104-015-0051-y
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发表时间：
2015
影响因子：
7
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Sun X;Wang Y;Chen B;Zhao X
用糖替代日粮淀粉对乳脂生产的积极影响被认为部分归因于抑制瘤胃反式-10生物氢化途径。然而，蔗糖是否以及如何抑制瘤胃反式-10生物氢化途径仍然是未知的。采用批式体外培养系统，研究了高精料日粮(料精比 = 40：60)中添加0(对照)、3%、6%和9%蔗糖的玉米淀粉对瘤胃发酵模式、脂肪酸(FA)生物氢化途径和与反式-11-反式-10生物氢化途径相关的细菌数量的影响。与对照组相比，用蔗糖替代玉米淀粉不会改变瘤胃pH值或总挥发性脂肪酸(VFA)的浓度，但对个体VFA的组成有显著影响。随着饲料中蔗糖浓度的增加，丁酸和戊酸的摩尔比例呈线性增加，而醋酸盐的摩尔比例呈二次曲线下降，丙酸、异丁酸和异戊酸的摩尔比例呈线性下降。此外，用蔗糖替代玉米淀粉导致C18：1反式-10的线性降低，C18：1反式-11、C18：2n-6和反式-11/反式-10的比例线性增加，C18：2n-6和C18：3n-3的生物加氢线性降低。随着饲料中蔗糖含量的增加，丁酸和CLA cis-9产生菌的数量增加，而cis-12产生菌Megasphaera elsdenii的数量显著减少。这些结果表明，在高精料中用蔗糖代替淀粉增加了丁酸的产量，并抑制了瘤胃反式-10生物氢化途径，这至少部分是由于纤维分离丁弧菌的丰度增加和埃尔斯登巨型弧菌的丰度降低所致。
The positive influence of replacing dietary starch with sugar on milk fat production has been proposed to be partially attributed to the inhibition of the rumen trans-10 biohydrogenation pathway. However, whether and how sucrose inhibits the rumen trans-10 biohydrogenation pathway remains elusive. A batch in vitro incubation system was used to evaluate effects of replacing cornstarch in a high-concentrate diet (forage to concentrate ratio = 40:60) with 0 (control), 3, 6 and 9 % of sucrose on rumen fermentation pattern, fatty acid (FA) biohydrogenation pathways and bacterial populations relating to trans-11 to trans-10 biohydrogenation pathways. Replacing dietary cornstarch with sucrose did not alter rumen pH or concentrations of total volatile fatty acids (VFA) in comparison with the control but significantly influenced the profiles of individual VFA. The molar proportions of butyrate and valerate were linearly increased, while that of acetate was quadratically decreased and those of propionate, isobutyrate and isovalerate were linearly decreased with increasing concentrations of sucrose in the diet. Furthermore, replacing cornstarch with sucrose led to a linear decrease in C18:1 trans-10, linear increases in the proportions of C18:1 trans-11, C18:2n-6 and the ratio of trans-11 to trans-10, and linear decreases in biohydrogenation of C18:2n-6 and C18:3n-3. The abundance of Butyrivibrio fibrisolvens, a butyrate and CLA cis-9, trans-11 producer, was increased with the increasing inclusion of sucrose in the diet, while the population of Megasphaera elsdenii, a CLA trans-10, cis-12 producer, was significantly decreased by all levels of sucrose replacements. These results indicate that replacing starch in a high-concentrate diet with sucrose increased butyrate production and inhibited the rumen trans-10 biohydrogenation pathway, which was at least partially due to increased abundance of Butyrivibrio fibrisolvens and decreased abundance of Megasphaera elsdenii.