DEVELOPMENTAL-CHANGES OF GANGLIOSIDE EXPRESSIONS IN POSTNATAL RAT CEREBELLAR CORTEX
DEVELOPMENTAL-CHANGES OF GANGLIOSIDE EXPRESSIONS IN POSTNATAL RAT CEREBELLAR CORTEX
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DOI：
10.1016/0006-8993(95)00923-e
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发表时间：
1995-11-27
期刊：
影响因子：
2.9
通讯作者：
TAI, T
中科院分区：
文献类型：
--
作者：
KOTANI, M;TERASHIMA, T;TAI, T
我们以前描述了神经节苷脂在成年大鼠脑内的不同分布，这是由特定的抗体检测到的。本文用鼠单抗免疫荧光技术研究了神经节苷脂在出生后大鼠小脑发育过程中的分布。使用了11种特异性识别每个神经节苷脂的单抗。我们的研究表明，在发育过程中，每个神经节苷脂的表达都发生了戏剧性的变化。GD3和O-Ac-GD3在出生后1、5和10日龄在小脑外颗粒层有较强的表达，而GD2在出生后5d最早出现在内颗粒层，GD1b在出生后早期弥漫在小脑皮质的各层。GD2和GD1b在20日龄、30日龄和80日龄在颗粒层表达较强，提示未成熟颗粒细胞表达GD3及其衍生物O-Ac-GD3，而成熟颗粒细胞表达GD2和GD1b。而GM1仅在第1天和第5天的外颗粒层和分子层中检测到。10d后，染色部位逐渐从外层向内颗粒层和白质扩散。外颗粒层和分子层的阳性细胞为Bergmann神经胶质细胞及其胞浆突起。这些特化星形胶质细胞的染色强度在出生后逐渐减弱。而GQ1b的表达在出生时很弱，随着发育逐渐增强，并在内颗粒层，尤其是成年期的小脑肾小球中检测到强烈表达，提示GQ1b的表达可能与突触相关的结构有关。其他神经节苷脂的表达在出生后的大鼠小脑中的表达也发生了发育变化。这些结果表明，特定的神经节苷脂可能在调节导致小脑皮质有序形成的早期事件中发挥重要作用。
We previously described the differential distribution of gangliosides in adult rat brain as detected by specific antibodies. We report here the distribution of gangliosides during the development of postnatal rat cerebellum by an immunofluorescence technique with mouse monoclonal antibodies (mAbs). Eleven mAbs that specifically recognize each ganglioside were used. Our study revealed that the expression of each ganglioside changed dramatically during the development. GD3 and O-Ac-GD3 were expressed intensely in the external granular layer at 1, 5, and 10 days, whereas GD2 was firstly detected in the internal granular layer at 5 days and GD1b was diffusely detected throughout all layers of the cerebellar cortex at early postnatal days. GD2 and GD1b were more intensely expressed in the granular layer at 20, 30, and 80 days, suggesting that premature granule cells express GD3 and its derivative, O-Ac-GD3, whereas mature granule cells express GD2 and GD1b intensely. On the other hand, GM1 was exclusively detected in the external granular layer and the molecular layer at 1 and 5 days. The staining sites spread gradually from these outer layers into the internal granular layer and the white matter after 10 days. The positive cells in the external granular layer and the molecular layer appeared to be Bergmann glial cells and their radially ascending cytoplasmic processes. The intensity of the staining in these specialized astroglial cells decreased gradually during postnatal days. In contrast, the expression of GQ1b was very faint at birth, but gradually increased during the development and was detected intensely in the internal granular layer, particularly in the cerebellar glomeruli in adulthood, suggesting that GQ1b expression may be associated with synapse-related structures. The developmental changes of the expression of other gangliosides were also recognized in the postnatal rat cerebellum. These results suggest that specific gangliosides may play an important role in regulating the early events responsible for the orderly formation of the cerebellar cortex.