Identification of a panel of five serum miRNAs as a biomarker for Parkinson's disease
Identification of a panel of five serum miRNAs as a biomarker for Parkinson's disease
复制标题
鉴定一组五种血清 miRNA 作为帕金森病的生物标志物。
DOI：
10.1016/j.parkreldis.2015.11.014
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发表时间：
2016-01-01
影响因子：
4.1
通讯作者：
Zhang, Junfeng
中科院分区：
文献类型：
--
作者：
Ding, Haixia;Huang, Zhen;Zhang, Junfeng
背景与目的：帕金森病（Parkinson's disease，PD）是仅次于阿尔茨海默病（Alzheimer's disease）的第二大年龄相关性神经退行性疾病。本研究的目的是确定血清miRNAs谱的差异是否可以区分PD患者和健康individuals.Methods：我们收集了106例散发性PD患者和91名年龄/性别匹配的健康对照者的血清样本。通过Solexa测序分析血清miRNA，然后进行qRT-PCR检查。qRT-PCR分析分为两个阶段，用于验证通过Solexa测序筛选的miRNA的表达。结果：在本研究中，我们建立了5种血清miRNAs的表达谱：miR-195表达上调，miR-185、miR-15 b、miR-221和miR-181 a表达下调。结论：这五种miRNA可以精确区分PD患者和健康个体，并可用作诊断PD的潜在血清生物标志物。(C)2015爱思唯尔有限公司版权所有。
Background and objective: Parkinson's disease (PD) is the second most common age-related neurodegenerative disorder after Alzheimer's disease. The aim of this work was to determine whether the differences of serum miRNAs profiling could distinguish PD patients from healthy individuals.Methods: We collected serum samples from 106 sporadic PD patients and 91 age/gender-matched healthy controls. Serum miRNAs were analysed by Solexa sequencing followed by a qRT-PCR examination. The qRT-PCR assay, which was divided into two phases, was used to validate the expression of miRNAs screened by Solexa sequencing. Receiver operating characteristic (ROC) curve analysis and clustering analysis were performed to determine the diagnostic usefulness of the selected miRNAs for PD.Results: In this study, we generated a profile of 5 serum miRNAs: miR-195 was up-regulated, and miR-185, miR-15b, miR-221 and miR-181a were down-regulated.Conclusion: This group of five miRNAs can precisely distinguish PD patients from health individuals and may be used as a potential serum-based biomarker for the diagnosis of PD. (C) 2015 Elsevier Ltd. All rights reserved.