Fluorescence Enhancement of a Microbial Rhodopsin via Electronic Reprogramming
Fluorescence Enhancement of a Microbial Rhodopsin via Electronic Reprogramming
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DOI：
10.1021/jacs.8b09311
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发表时间：
2019-01-09
影响因子：
15
通讯作者：
Olivucci, Massimo
中科院分区：
文献类型：
--
作者：
Marin, Maria del Carmen;Agathangelou, Damianos;Olivucci, Massimo
荧光增强型微生物视紫红质基因工程在光遗传学领域具有重要意义。在这里，我们报告的发现，两个突变体（W76 S/Y179 F和L 83 Q）的感觉视紫红质从蓝藻鱼腥藻PCC 7120具有相反的荧光行为。事实上，虽然W76 S/Y179 F显示，相对于野生型蛋白，红光发射增加了近10倍，但第二种不发射。因此，W76 S/Y179 F，L 83 Q对提供了一个前所未有的机会，在微生物视紫红质，这是追求结合瞬态吸收光谱和多构型量子化学的荧光增强的调查。这种调查的结果点的异构化阻断电子效应的直接原因的瞬时（亚皮秒）荧光增强。
The engineering of microbial rhodopsins with enhanced fluorescence is of great importance in the expanding field of optogenetics. Here we report the discovery of two mutants (W76S/Y179F and L83Q) of a sensory rhodopsin from the cyanobacterium Anabaena PCC7120 with opposite fluorescence behavior. In fact, while W76S/Y179F displays, with respect to the wild-type protein, a nearly 10-fold increase in red-light emission, the second is not emissive. Thus, the W76S/Y179F, L83Qpair offers an unprecedented opportunity for the investigation of fluorescence enhancement in microbial rhodopsins, which is pursued by combining transient absorption spectroscopy and multiconfigurational quantum chemistry. The results of such an investigation point to an isomerization-blocking electronic effect as the direct cause of instantaneous (subpicosecond) fluorescence enhancement.