Creatine supplementation reduces sleep need and homeostatic sleep pressure in rats.
Creatine supplementation reduces sleep need and homeostatic sleep pressure in rats.
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DOI：
10.1111/jsr.12523
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发表时间：
2017-06
影响因子：
4.4
通讯作者：
Basheer R
中科院分区：
文献类型：
--
作者：
Dworak M;Kim T;Mccarley RW;Basheer R
睡眠被认为可以促进大脑能量的恢复。目前尚不清楚增加大脑内的能量可用性是否会减少睡眠需求。胍氨基酸肌酸（Cr）是细胞能量稳态中众所周知的能量助推器。口服肌酸一水合物补充剂（CS）可提高运动表现，并已被证明对认知能力、神经保护和昼夜节律有实质性影响。CS对细胞高能分子和睡眠-觉醒行为的影响尚不清楚。在这里，我们检查了大鼠口服CS四周前后的睡眠-觉醒行为和大脑能量代谢。CS显着减少总睡眠时间和非快速眼动（NREM）睡眠期间的光（不活跃），但不是在黑暗（活跃）期间。睡眠剥夺6 h后，CS大鼠NREM睡眠和NREM δ活动显著降低。脑能量代谢物的生化分析显示，CS后磷酸肌酸的趋势增加，而细胞ATP水平下降。微透析分析表明，睡眠剥夺引起的细胞外腺苷的增加在CS后减弱。这些结果表明，CS减少睡眠需要和稳态睡眠压力的大鼠，从而表明其在治疗睡眠相关疾病的潜力。
Sleep has been postulated to promote brain energy restoration. It is yet unknown if increasing the energy availability within the brain reduces the sleep need. The guanidine amino acid creatine (Cr) is a well-known energy booster in cellular energy homeostasis. Oral creatine-monohydrate supplementation (CS) increases exercise performance and has been shown to have substantial effects on cognitive performance, neuroprotection and circadian rhythms. The effect of CS on cellular high energy molecules and sleep-wake-behavior is unclear. Here, we examined the sleep-wake behavior and brain energy metabolism before and after four week-long oral administration of CS in the rat. CS significantly decreased total sleep time and non-rapid eye movement (NREM) sleep during the light (inactive) but not during the dark (active) period. NREM-sleep and NREM delta activity were significantly decreased in CS rats after 6h of sleep deprivation. Biochemical analysis of brain energy metabolites showed a tendency increase in phosphocreatine after CS, while cellular ATP level decreased. Microdialysis analysis showed that the sleep deprivation-induced increase in extracellular adenosine was attenuated after CS. These results suggest that CS reduces sleep need and homeostatic sleep pressure in rats, thereby indicating its potential in the treatment of sleep related disorders.