EXPERIMENTAL STUDIES ON BONE-INDUCING ACTIVITY OF COMPOSITES OF ATELOPEPTIDE TYPE-I COLLAGEN AS A CARRIER FOR ECTOPIC OSTEOINDUCTION BY RHBMP-2
EXPERIMENTAL STUDIES ON BONE-INDUCING ACTIVITY OF COMPOSITES OF ATELOPEPTIDE TYPE-I COLLAGEN AS A CARRIER FOR ECTOPIC OSTEOINDUCTION BY RHBMP-2
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DOI：
10.1006/bbrc.1995.1340
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发表时间：
1995-03-08
影响因子：
3.1
通讯作者：
IIZUKA, T
中科院分区：
文献类型：
--
作者：
FUJIMURA, K;BESSHO, K;IIZUKA, T
以新鲜猪皮为载体，研究了重组人骨形态发生蛋白-2（rhBMP-2）异位诱导成骨的作用。检查了四个治疗组（N=5）：仅植入端肽I型胶原的对照组，以及分别将端肽I型胶原与2、10、50 μ g rhBMP-2植入10周龄Wistar大鼠的小腿肌肉中的组II、III和IV。植入4周后，进行软X线和光镜检查。此外，钙（Ca）含量和碱性磷酸酶（ALP）活性进行了评价。发现植入区域（组II、III和IV）有新骨形成。即使在2 μ g rhBMP-2（组II）的植入区域也能诱导骨形成，其程度取决于rhBMP-2的剂量。这些结果表明，端肽I型胶原蛋白是一种有效的载体异位骨诱导rhBMP-2，并可能作为载体rhBMP在重建手术骨缺损和增强。(C)出版社：Academic Press
Atelopeptide type I collagen derived from fresh porcine skin was evaluated as a carrier for ectopic osteoinduction by recombinant human bone morphogenetic protein-2 (rhBMP-2) in rats. Four treatment groups (N=5) were examined: a control group in which only atelopeptide type I collagen was implanted, and groups II, III and IV in which atelopeptide type I collagen with 2, 10, 50 mu g of rhBMP-2 was implanted in to the calf muscles of 10-week-old Wistar rats, respectively. Four weeks after the implantation, soft X-ray and light microscopic examinations were performed. In addition, calcium (Ca) content and alkaline phosphatase (ALP) activity were evaluated. New bone formation in the implanted regions (groups II, III and IV) were revealed. Bone formation was induced in the implanted region of even 2 mu g of rhBMP-2 (group II), and its degree was dependent on the dose of rhBMP 2. These results indicate that atelopeptide type I collagen is an effective carrier for ectopic osteoinduction by rhBMP-2 and may act as a carrier for rhBMP in reconstructive surgery for bony defect and augmentation. (C) 1995 Academic Press, Inc.