Green tea polyphenol extract attenuates inflammation in interleukin-2-deficient mice, a model of autoimmunity
Green tea polyphenol extract attenuates inflammation in interleukin-2-deficient mice, a model of autoimmunity
复制标题
DOI：
10.1093/jn/131.7.2034
复制
发表时间：
2001-07-01
影响因子：
4.2
通讯作者：
McClain, CJ
中科院分区：
文献类型：
--
作者：
Varilek, GW;Yang, FJ;McClain, CJ
绿色茶多酚（GrTP）以前已被证明可以减少内毒素诱导的肿瘤坏死因子-ct的产生和小鼠的死亡率。我们目前的研究表明，GrTP抑制炎症反应，并可能是有用的，在治疗慢性炎症stales，如炎症性肠病。在这项初步研究中，我们检查了GrTP是否降低白细胞介素-2缺陷（IL-2（-/-）小鼠的疾病活动。8周龄IL-2（-/-）C57 BL/6 J小鼠随机分为两组，分别给予5 g/L的GrTP水和对照水。1周后，从小鼠亚组建立外植体结肠和固有层淋巴细胞（LPL）培养物，并收集上清液。与对照组相比，GrTP处理小鼠的培养上清液显示自发性干扰素-γ和肿瘤坏死因子-α分泌减少。在第6周，GrTP组结肠炎的严重程度较低，表现为较低的组织学评分和湿结肠重量。这与血清淀粉样蛋白A的血浆水平较低、体重增加和红细胞压积改善有关。这些结果表明，GrTP减弱了11-2（-/-）小鼠的炎症，并表明GrTP在治疗慢性炎症性疾病如炎症性肠病中的作用。
Green tea polyphenols (GrTP) have been previously shown to decrease endotoxin-induced tumor necrosis factor-ct production and lethality in mice. Our present studies demonstrate that GrTP inhibit inflammatory responses and may be useful in treating chronic inflammatory stales, such as inflammatory bowel disease. In this preliminary study, we examined whether GrTP decrease disease activity in interleukin-2-deficient (IL-2(-/-) mice. Eight-week old IL-2(-/-) C57BL/6J mice who were fed nonpurified diet were randomly assigned to receive water with GrTP (5 g/L) or water alone (control) for up to 6 wk. After 1 wk, explant colon and lamina propria lymphocyte (LPL) cultures were established from a subgroup of mice and supernatants collected. Culture supernatants from GrTP-treated mice showed decreased spontaneous interferon-gamma and tumor necrosis factor-alpha secretion compared with that of controls. At 6 wk, the GrTP group had less severe colitis as demonstrated by lower histologic scores and wet colon weights. This was associated with lower plasma levels of serum amyloid A, increased weight gain and improved hematocrits. These results show that GrTP attenuated inflammation in 11-2(-/-) mice and suggest a role for GrTP in treating chronic inflammatory diseases such as inflammatory bowel disease.