Strategies for efficiency and accuracy in gene expression
Strategies for efficiency and accuracy in gene expression
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基因表达效率和准确性的策略
DOI：
10.1016/0968-0004(87)90060-0
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发表时间：
1987
影响因子：
13.8
通讯作者：
C. Kurland
中科院分区：
文献类型：
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作者：
C. Kurland
近年来，人们对基因表达机制的认识出现了一些问题。结果表明，所有的模型系统研究都表明，核苷基的结合基不是完全立体特异性的，以支持其他核苷基或翻译基的准确性。Hopfield-Niruo底物校对模型的失败使基因表达的准确性从一个方面得到了保证，但它产生了另一组问题。从低保真度的基本过程（如碱基规划）中获得精度的方法是：重复基本步骤的足够数量的符文。由于对允许的重复数量没有明确的规定，因此对可实现的精度没有明确的规定，然后设置基因表达的错误率。三节：这一系列的三个节；在本文中，我们将介绍一种用于翻译装置的基因编码效率的缺陷，然后将一个简单的基因编码程序作为工具来探索使用不同的编码策略来指定在不同生长条件下选择性表达的蛋白质序列的后果。并探讨了平移速度的精度。本文将引入误差余弦和准确度余弦中的一种，用于解释跨位精度改变的突变体的生长表型。将跨位误差的相关成本与第三段的新闻误差的相关成本进行比较。本文将引入翻译存在物理约束的概念，并提出氨酰基- trna选择的准确性与信使RNA表达的准确性之间存在反比关系，这一反比关系将为细菌药物依赖的性质提供一些线索，同时也将提出这种反比关系。特别是，我认为，至少在一定程度上，误义错误率的较低水平是由可容忍的mRNA易位子错误的较高水平决定的。或者，易位子的准确性可能受到与tRNA错误的较高准确性相关的laneac co的限制
Recently there has been a sh~ m the perception of problems assoaated with the mechanisms of gene expression Formerly it seemed that the problem i~. s to da'cover where the remarkable accuracy of gene expresaon ongmated The my~ tery i~ s that all model systemstudtes suggested that the binding mterac~ ons ofnucleoades are not suflfiaen@ stereo-speafic to support the aca~ racy ofather IranscnpRon or oftranslaa~ z The maodactlon of the Hopfield-Niruo model of substrate proofreading demysafied ltm aspect of the accuracy of gene expression, but tt generated another set of problems Thus, the way to get/ugh accuracy out of a low fidehty elementary process such as base pmnng: s to repeat the elementary step a sufficient number of runes Since there is no pnnaple Imm on the nmnber of allowable repenaons, there ts no prmaple lunu on the auamable accuracy What then sets the error rates ofgene expre. sston~ This series of three arncles; i~ ll address this quesvon In this first aracle, the notch of a na2xmuzed kmeac effiaeney for the translaaon apparatus~ ll be introduced A sunple opnrruzanon prmaple wdl then be used as a tool to explore the consequences of using ckfferent coding sirategzes to speafy the sequences ofprotems that are etpressed selecavely under different growth condmons In the second aracle the antagomsac relat~ nshlp bea,, een lanenc c 3, and accuracy of translanon v~ dl be explored The nonon of a lanenc optm uzau. on of the cos~ of errors and the cos~ of accuracy wdl be mtrodaced and used to explain the growth phenotypes ofmutants with altered translanonal accurat 3, The costs assocurled w~ th errors of translocanon wdl be contrasted wtth those mst~ ated wch nusscnse errors m the thtrd aracle In addmon,! wdl introduce the notion that there ts a physical constraint m translaaon tl~: mposes a reoprocal relanonstnp between the accuracy of ammoacyl-tRNA selecnon and the accuracy of messenger RNA tnovemenl Th~ s reciprocal relaaottshtp wdl provtde some ms: ght mto the nature of drug dependence m bactena and,,~ ll also suggest the otahne.~ of an answer to our original queslton In paracular, I wdl suggest that the lower level of the nussense error rate ts determined, at least m pan, by the upper level of mRNA translocanon errors that~ tolerable Ltkeiwse, the accuracy of translocanon may be constrained by the laneac co~ assoaated with etcesstve accuracy of tRNA sdecnon