Cellulose Acetate Electrophoresis of Hemoglobin.
Cellulose Acetate Electrophoresis of Hemoglobin.
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DOI：
10.1007/978-1-4939-8793-1_7
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发表时间：
2018-11
影响因子：
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通讯作者：
Ramesh Kumar;Wilbert A. Derbigny
中科院分区：
文献类型：
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作者：
Ramesh Kumar;Wilbert A. Derbigny
醋酸纤维素膜上的电泳由于其简单性而被最广泛地使用，并且除了电泳装置和醋酸纤维素条之外不使用任何复杂的仪器。在这里，我们描述了一个改进的版本的醋酸纤维素膜电泳分离血红蛋白的血液样品。用这种方法可以得到尖锐、清晰、无拖尾效应的条带。本文所述的方法和装置将适合于在电极之间测量的高达60 V/cm的电压下在1小时内分离蛋白质级分。
The electrophoresis on cellulose acetate membrane is most widely used because of its simplicity, and is without the use of any sophisticated instrument other than electrophoresis apparatus and the cellulose acetate strip. Here we describe a modified version of cellulose acetate membrane electrophoresis for hemoglobin separation from blood sample. Sharp, clear bands without tailing effects can be obtained with this method. The method and apparatus described here would be appropriate to separate protein fractions under 1 h at voltages up to 60 V/cm measured between the electrodes.