Proteins interacting with Lin1p, a putative link between chromosome segregation, mRNA splicing and DNA replication in Saccharomyces cerevisiae
Proteins interacting with Lin1p, a putative link between chromosome segregation, mRNA splicing and DNA replication in Saccharomyces cerevisiae
复制标题
DOI：
10.1002/yea.919
复制
发表时间：
2002-11-01
期刊：
影响因子：
2.6
通讯作者：
Kurlandzka, A
中科院分区：
文献类型：
--
作者：
Bialkowska, A;Kurlandzka, A
在有丝分裂过程中参与染色体分离的蛋白质可能参与其他细胞周期协调过程。使用双杂交筛选，我们确定了一种新的核蛋白，林1，相互作用的Irr 1 p/Scc 3 p，一个组成部分的cohesin复合物。以Lin 1 p为诱饵的第二轮双杂交试验鉴定出6个蛋白：Prp 8、Slx 5、Siz 2、Wss 1、Rfc 1和YIL 149 w。这些蛋白质先前已被证明参与mRNA剪接，DNA复制，染色体凝聚，染色单体分离和选择性凝聚。我们建议，Lin 1 p可能构成这些过程之间的联系。版权所有（C）2002约翰威利父子有限公司
Proteins involved in chromosome segregation during mitosis are likely to participate in other cell cycle-coordinated processes. Using a two-hybrid screen we identified a novel nuclear protein, Lin1, interacting with Irr1p/Scc3p, a component of the cohesin complex. The second round of two-hybrid assay with Lin1p as the bait resulted in the identification of six proteins: Prp8, Slx5, Siz2, Wss1, Rfc1 and YIL149w. These proteins have previously been shown to participate in mRNA splicing, DNA replication, chromosome condensation, chromatid separation and alternative cohesion. We propose that Lin1p may constitute a link among these processes. Copyright (C) 2002 John Wiley Sons, Ltd.