Direct intracellular measurement of deoxygenated hemoglobin S solubility
Direct intracellular measurement of deoxygenated hemoglobin S solubility
复制标题
DOI：
10.1182/blood.v98.3.883
复制
发表时间：
2001-08-01
期刊：
影响因子：
20.3
通讯作者：
Suzuka, SM
中科院分区：
文献类型：
--
作者：
Fabry, ME;Desrosiers, L;Suzuka, SM
脱氧血红蛋白S （HbS）的溶解度，即完全脱氧血红蛋白S与聚合物平衡的浓度（C-SAT），是决定体内聚合物形成的一个因素。然而，C-SAT的测量通常在远离生理的条件下进行。Benesch等人对HbS的溶液研究表明，p50，即血红蛋白与氧半饱和的点，与形成的聚合物数量成正比，可以用来测量C-SAT。该方法已扩展到通过改变细胞外渗透压来测量完整红细胞中的C-SAT，而细胞外渗透压反过来又改变细胞内血红蛋白浓度。该方法在红细胞含量完整的生理条件下测量细胞内C-SAT，可应用于人和转基因小鼠红细胞。通过测量AA、SS和as红细胞细胞外渗透压的p50来证明这一原理。(C) 2001年由美国血液学会出版。
The solubility of deoxygenated hemoglobin S (HbS), which is the concentration of fully deoxygenated HbS in equilibrium with polymer (C-SAT), is a factor that determines in vivo polymer formation. However, measurement of C-SAT is usually performed under conditions that are far from physiological. In solution studies of HbS by Benesch at al, it was demonstrated that p50, the point at which hemoglobin is half-saturated with oxygen, is proportional to the amount of polymer formed and that it may be used to measure C-SAT. This method has been extended to measure C-SAT In Intact red cells by varying extracellular osmolarity, which, in turn, varies intracellular hemoglobin concentration. This method measures intracellular C-SAT under physiological conditions with intact red cell contents and can be applied to human and transgenic mouse red cells. The principle is demonstrated by measuring p50 as a function of extracellular osmolarity for AA, SS, and AS red cells. (C) 2001 by The American Society of Hematology.