In vitro assays for detecting carcinogenic mineral fibres: a comparison of two assays and the role of fibre size.
In vitro assays for detecting carcinogenic mineral fibres: a comparison of two assays and the role of fibre size.
复制标题
检测致癌矿物纤维的体外测定：两种测定的比较以及纤维尺寸的作用。
DOI：
10.1093/carcin/7.12.1971
复制
发表时间：
1986
期刊：
影响因子：
4.7
通讯作者：
K. Donaldson
中科院分区：
文献类型：
--
作者：
G. M. Brown;H. Cowie;J. M. Davis;K. Donaldson
使用 V79/4 和 A549 细胞在两个体外测试系统中比较了 13 种致癌纤维粉尘样品的活性。其中 11 个粉尘在两项检测中具有相似的等级，但两个样本的结果不同。检查了 12 个样品的纤维长度和直径分布及其在每次测定中的活性之间的关系。两种测定中纤维长度和生物活性之间都存在显着关联。这种关系表明，随着纤维长度的增加，关联强度也随之增加。在 A549 测定中，纤维直径和体外活性之间唯一显着的关联是通过大于 0.2 微米的纤维来证明的。
The activities of 13 carcinogenic fibrous dust samples were compared in two in vitro test systems using V79/4 and A549 cells. Eleven of the dusts had similar ranks in both assays but two samples had disparate results. The relationship between the fibre length and diameter distributions of 12 of the samples and their activity in each assay was examined. A significant association between fibre length and biological activity occurred in both assays. This relationship showed increasing strength of association with increasing fibre length. The only significant association between fibre diameter and activity in vitro was demonstrated with fibres greater than 0.2 micron in the A549 assay.