Insulin and insulin-like growth factor-I receptor mediated differentiation of 3T3-F442A cells into adipocytes: Effect of PI 3-kinase inhibition
Insulin and insulin-like growth factor-I receptor mediated differentiation of 3T3-F442A cells into adipocytes: Effect of PI 3-kinase inhibition
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DOI：
10.1006/bbrc.1998.8637
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发表时间：
1998-05-19
影响因子：
3.1
通讯作者：
Joshi, RL
中科院分区：
文献类型：
--
作者：
Christoffersen, CT;Tornqvist, H;Joshi, RL
在各种激素浓度下检测胰岛素和胰岛素样生长因子（IGF-I和IGF-II）诱导3 T3-F442 A细胞分化为脂肪细胞的能力。胰岛素和IGFs在与其同源受体结合相容的浓度下促进分化，表明胰岛素和EGF-I受体都能够促进这种分化。在PI 3-激酶的特异性抑制剂LY 294002的存在下，3 T3-F442 A细胞的脂肪细胞转化被完全阻断，表明PI 3-激酶活性在触发该分化过程的初始信号事件中起着至关重要的作用。(C)北京：科学出版社.
The ability of insulin and insulin-like growth factors (IGF-I and IGF-II) to induce differentiation of 3T3-F442A cells into adipocytes was examined at various hormone concentrations. Both insulin and the IGFs promoted differentiation at concentrations compatible with binding to their cognate receptors, suggesting that both insulin and EGF-I receptors are capable of promoting this differentiation. Adipocyte conversion of 3T3-F442A cells was completely blocked in the presence of LY294002, a specific inhibitor of PI 3-kinase, indicating that PI 3-kinase activity plays a crucial role in the initial signalling events that trigger this differentiation process. (C) 1998 Academic Press.