Enzyme-based assay for quantification of UDP-GlcNAc in cells and tissues.
Enzyme-based assay for quantification of UDP-GlcNAc in cells and tissues.
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DOI：
10.1016/j.crmeth.2023.100537
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发表时间：
2023-07-24
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在本期《细胞报告方法》中，桑登等人开发了一种酶促检测方法，用于测量细胞和组织中的UDP - N - 乙酰葡糖胺（UDP - GlcNAc）水平。通过报告底物本身的水平，这种方法有可能增进该领域对多种条件下UDP - GlcNAc浓度的了解。
在本期《细胞报告方法》中，桑登等人开发了一种酶促检测方法，用于测量细胞和组织中的UDP - N - 乙酰葡糖胺（UDP - GlcNAc）水平。通过报告底物本身的水平，这种方法有可能增进该领域对多种条件下UDP - GlcNAc浓度的了解。
In this issue of Cell Reports Methods, Sunden et al. develop an enzymatic assay to measure UDP-GlcNAc levels from cells and tissue. By reporting on the level of the substrate itself, this approach can potentially enhance the fields’ understanding of UDP-GlcNAc concentration under a variety of conditions. In this issue of Cell Reports Methods, Sunden et al. develop an enzymatic assay to measure UDP-GlcNAc levels from cells and tissue.1 By reporting on the level of the substrate itself, this approach can potentially enhance the fields’ understanding of UDP-GlcNAc concentration under a variety of conditions.