Scientific Opinion on the use of a gamma interferon test for the diagnosis of bovine tuberculosis
Scientific Opinion on the use of a gamma interferon test for the diagnosis of bovine tuberculosis
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DOI：
10.2903/j.efsa.2012.2975
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发表时间：
2012-12-01
期刊：
影响因子：
3.3
通讯作者：
Zientara, Stephan
中科院分区：
文献类型：
--
作者：
Authie, Edit;Berg, Charlotte;Zientara, Stephan
获得、维持、暂停、撤销或重新获得官方无结核病牛群地位以及联盟内部贸易认证的程序均以结核菌素皮肤测试的结果为基础。皮肤测试有许多缺点，因此评估了伽马干扰素测试和欧盟立法中纳入的其他测试的适用性。适用性是指该检测的灵敏度相当于或优于欧盟目前使用的标准检测，特异性不低于欧盟目前使用的特异性最低的标准检测。此外，不应存在可能影响测试性能的可预见的实际困难。结论是，基于纯化蛋白衍生物的γ干扰素测试可以包含在官方测试中，以证明自由。然而，一些结果表明，基于纯化蛋白衍生物的γ干扰素测试的特异性可能并不总是与单一皮内结核菌素测试一样高。如果包含该测试，则欧盟应协调用于此目的的协议。根据审查的信息，尚不应考虑将其他测试纳入官方测试中，以授予和保留官方无结核病牛群状态。进一步评估γ干扰素试验的适用性应研究环境分枝杆菌的存在、牛群中牛结核病的流行情况、动物的年龄类型和牛结核病试验史等因素的影响，所有这些都可能影响试验的特异性，从而影响试验在不同情况下证明牛结核病不存在的适用性。 (C) 欧洲食品安全局，2012 年。
The procedures for gaining, maintaining, suspending, withdrawing or re-gaining official bovine tuberculosis free herd status and for certification for intra-Union trade are based on the results of tuberculin skin tests. The skin test has a number of drawbacks, therefore the suitability of the gamma interferon test and other tests to be included by EU legislation was assessed. Suitability means that the test has a sensitivity equivalent or superior to the standard test currently used in the European Union and specificity not lower than that of the standard test with the lowest specificity used in the EU. Furthermore, there should be no foreseeable practical difficulties that could compromise test performance. It was concluded that purified protein derivative based gamma interferon tests can be included amongst the official tests for the purpose of demonstrating freedom. However, some results suggest that the specificity of the purified protein derivative based gamma interferon tests may not always be as high as the single intradermal tuberculin test. In case the test is included, the protocols for its use for this purpose should be harmonised in the EU. Based on the reviewed information, other tests should not yet be considered for inclusion in the official tests for the purpose of granting and retaining official tuberculosis free herd status. Further evaluation of the suitability of the gamma interferon tests test should study the influence of factors such as the presence of environmental mycobacteria, prevalence of bovine tuberculosis in the herd, the age type and bovine tuberculosis test history of the animals all of which may affect test specificity and hence the suitability of the test for demonstrating freedom from bovine tuberculosis in different situations. (C) European Food Safety Authority, 2012.