Storage of Erythrocytes Induces Suicidal Erythrocyte Death
Storage of Erythrocytes Induces Suicidal Erythrocyte Death
复制标题
DOI：
10.1159/000445657
复制
发表时间：
2016-01-01
影响因子：
--
通讯作者：
Lang, Philipp A.
中科院分区：
文献类型：
--
作者：
Lang, Elisabeth;Pozdeev, Vitaly I.;Lang, Philipp A.
背景/目的：与有核细胞的凋亡类似，红细胞（RBC）也会发生自杀性细胞死亡，称为红细胞凋亡。它的特点是细胞收缩和磷脂酰丝氨酸易位。由于非选择性阳离子通道的激活，细胞内钙浓度的增加会引发红细胞增生。促红细胞生成素抑制阳离子通道，从而抑制红细胞凋亡。红细胞被巨噬细胞吞噬，因此迅速从循环血液中清除。在这项研究中，我们探讨了红细胞的储存是否会影响红血球的发生率。方法：用流式细胞术定量膜联蛋白V结合的磷脂酰丝氨酸暴露红细胞和荧光-3的胞质Ca2+活性。通过注射羧基荧光素琥珀酰亚胺酯（CBE）标记的红细胞来检测小鼠红细胞的清除。结果：保存42天显著增加了磷脂酰丝氨酸暴露和溶血红细胞的百分比，这一作用因细胞外钙的去除而减弱。添加促红细胞生成素可抑制磷脂酰丝氨酸暴露。输血后，与储存2天相比，储存10天后，循环血液中小鼠cfsf标记红细胞的清除率明显更高。结论：红细胞储存通过Ca2+和促红细胞生成素敏感机制触发红细胞凋亡。(C) 2016，作者：s . Karger AG，巴塞尔出版
Background/Aims: Similar to apoptosis of nucleated cells, red blood cells (RBC) can undergo suicidal cell death - called eryptosis. It is characterized by cell shrinkage and phosphatidylserine translocation. Eryptosis is triggered by an increase of intracellular calcium concentration due to activation of nonselective cation channels. The cation channels and consequently eryptosis are inhibited by erythropoietin. Fryptotic RBC are engulfed by macrophages and thus rapidly cleared from circulating blood. In this study, we explored whether storage of RBC influences the rate of eryptosis. Methods: Flow cytometry was employed to quantify phosphatidylserine exposing erythrocytes from annexin V binding and cytosolic Ca2+ activity from Fluo-3 fluorescence. Clearance of stored murine RBC was tested by injection of carboxyfluorescein succinimidyl ester (CBE) -labelled erythrocytes. Results: Storage for 42 days significantly increased the percentage of phosphatidylserine exposing and haemolytic erythrocytes, an effect blunted by removal of extracellular calcium. Phosphatidylserine exposure could be inhibited by addition of erythropoietin. Upon transfusion, the clearance of murine CFSF-labelled RBC from circulating blood was significantly higher following storage for 10 days when compared to 2 days of storage. Conclusion: Storage of RBC triggers eryptosis by Ca2+ and erythropoietin sensitive mechanisms. (C) 2016 The Author(s) Published by S. Karger AG, Basel