SEVERAL RECEPTOR TYROSINE KINASE GENES OF THE EPH FAMILY ARE SEGMENTALLY EXPRESSED IN THE DEVELOPING HINDBRAIN
SEVERAL RECEPTOR TYROSINE KINASE GENES OF THE EPH FAMILY ARE SEGMENTALLY EXPRESSED IN THE DEVELOPING HINDBRAIN
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DOI：
10.1016/0925-4773(94)90091-4
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发表时间：
1994-07-01
影响因子：
2.6
通讯作者：
GILARDIHEBENSTREIT, P
中科院分区：
文献类型：
--
作者：
BECKER, N;SEITANIDOU, T;GILARDIHEBENSTREIT, P
后脑中的模式形成涉及导致形成同色异谱单位的分割过程，表现为被称为菱体（r）的连续的脑区。在寻找基因参与细胞间的相互作用，在后脑分割，我们已经筛选出蛋白激酶基因与限制表达模式，在这一地区的中枢神经系统。我们提出了三个新的小鼠基因，Sek-2，Sek-3和Sek-4（推定的跨膜受体蛋白酪氨酸激酶（RTK）的Eph亚家族成员）的克隆，其染色体位置的鉴定，并分析其7.5和10.5天的发展之间的表达。在形态分割之前，Sek-2在对应于预期的R4和相邻的中胚层的横向条纹中转录，这表明可能在后脑分割和神经上皮和中胚层之间的信号传导中起作用。Sek-3和Sek-4具有共同的表达结构域，包括r3、r5和中脑的一部分，以及间脑、端脑、脊髓和中胚层和神经嵴衍生物中的特异性结构域。连同我们先前发现Sek（Sek-1）在r3和r5中表达（Gilardi-Hebenstreit et al.，1992; Nieto等人，1992），这些数据表明RTK的Eph家族的成员可能在后脑的节段性模式中合作。
Pattern formation in the hindbrain involves a segmentation process leading to the formation of metameric units, manifested as successive swellings known as rhombomeres (r). In search for genes involved in cell-cell interactions during hindbrain segmentation, we have screened for protein kinase genes with restricted expression patterns in this region of the CNS. We present the cloning of three novel mouse genes, Sek-2, Sek-3 and Sek-4 (members of the Eph subfamily of putative transmembrane receptor protein tyrosine kinases (RTKs)), the identification of their chromosomal locations, and the analysis of their expression between 7.5 and 10.5 days of development. Before morphological segmentation, Sek-2 is transcribed in a transverse stripe corresponding to prospective r4 and the adjacent mesoderm, suggesting possible roles both in hindbrain segmentation and signalling between neuroepithelium and mesoderm. Sek-3 and Sek-4 have common domains of expression, including r3, r5 and part of the midbrain, as well as specific domains in the diencephalon, telencephalon, spinal cord and in mesodermal and neural crest derivatives. Together with our previous finding that Sek (Sek-1) is expressed in r3 and r5 (Gilardi-Hebenstreit et al., 1992; Nieto et al., 1992), these data indicate that members of the Eph family of RTKs may co-operate in the segmental patterning of the hindbrain.