The role of selective transport in neuronal protein sorting
The role of selective transport in neuronal protein sorting
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DOI：
10.1016/s0896-6273(00)81178-2
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发表时间：
2000-05-01
期刊：
影响因子：
16.2
通讯作者：
Banker, G
中科院分区：
文献类型：
--
作者：
Burack, MA;Silverman, MA;Banker, G
为了评估是否选择性微管为基础的囊泡运输神经元蛋白的极化分布的基础上，我们表达了绿色荧光蛋白（GFP）标记的嵌合体的代表性轴突和树突状细胞膜蛋白在培养的海马神经元和可视化的载体囊泡含有这些蛋白在活细胞中的运输。含有树突状蛋白，转铁蛋白受体（TfR）的囊泡，优先运输到树突和轴突排除。相反，含有轴突蛋白NgCAM（神经胶质细胞粘附分子）的囊泡被运输到树突和轴突中。这些数据表明，神经元利用两种不同的机制为目标的极化膜蛋白，一个（树突状蛋白）的基础上选择性运输，其他（轴突蛋白）的基础上的选择性“过滤器”，发生在运输的下游。
To assess whether selective microtubule-based vesicle transport underlies the polarized distribution of neuronal proteins, we expressed green fluorescent protein- (GFP-) tagged chimeras of representative axonal and dendritic membrane proteins in cultured hippocampal neurons and visualized the transport of carrier vesicles containing these proteins in living cells. Vesicles containing a dendritic protein, transferrin receptor (TfR), were preferentially transported into dendrites and excluded from axons. In contrast, vesicles containing the axonal protein NgCAM (neuron-glia cell adhesion molecule) were transported into both dendrites and axons. These data demonstrate that neurons utilize two distinct mechanisms for the targeting of polarized membrane proteins, one (for dendritic proteins) based on selective transport, the other (for axonal proteins) based on a selectivity "filter" that occurs downstream of transport.