STAINING FOR MICROVASCULAR ALKALINE-PHOSPHATASE IN THICK CELLOIDIN SECTIONS OF NERVOUS-TISSUE - MORPHOMETRIC AND PATHOLOGICAL APPLICATIONS
STAINING FOR MICROVASCULAR ALKALINE-PHOSPHATASE IN THICK CELLOIDIN SECTIONS OF NERVOUS-TISSUE - MORPHOMETRIC AND PATHOLOGICAL APPLICATIONS
复制标题
DOI：
10.1016/0026-2862(84)90053-0
复制
发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
SCARROW, WG
中科院分区：
文献类型：
--
作者：
BELL, MA;SCARROW, WG
碱性磷酸酶在包括人在内的许多动物的中枢和外周神经系统的毛细血管（通常是小动脉）的内皮中是有活性的。将这种酶的改良金属染色剂应用于脑或神经的厚切片（50-500 μ m），产生微血管系统的显著混合物。可通过光学显微镜或微焦点射线照相术检查制剂。厚切片（特别是在100 μ m）适合于在光学显微镜水平上的血管形态测定。材料的火棉胶包埋允许样品的储存，并确保粘性切片可以连续切割，标记和轻松处理。复染可以显示与血管相关的实质的正常和病理特征。制备和形态测定技术进行了描述。例子来自海马和距状核微血管及其与正常衰老和阿尔茨海默型老年性痴呆中老年斑的关系的当前研究。
Alkaline phosphatase enzyme is active in the endothelium of capillaries (and often arterioles) in the central and peripheral nervous systems of many animals including man. A modified metallic stain for this enzyme applied to thick (50-500 .mu.m) sections of brain or nerve produces a striking mixture of the microvascular system. Preparations may be examined by light microscopy or microfocal radiography. Thick sections (particularly at 100 .mu.m) are suitable for vascular morphometry at the light microscopic level. Celloidin embedding of the material permits storage of the specimen and ensures that cohesive sections can be cut serially, labeled and handled with ease. Counterstaining permits normal and pathological features of the parenchyma to be demonstrated in relation to the vessels. Techniques of preparation and morphometry are described. Examples are drawn from current studies of the hippocampal and calcarine microvasculatures and their relation to senile plaques in normal aging and senile dementia of the Alzheimer type.