ALTERED KERATIN EXPRESSION IN BENIGN AND MALIGNANT SKIN DISEASES REVEALED WITH MONOCLONAL-ANTIBODIES
ALTERED KERATIN EXPRESSION IN BENIGN AND MALIGNANT SKIN DISEASES REVEALED WITH MONOCLONAL-ANTIBODIES
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DOI：
10.1097/00000372-198906000-00002
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发表时间：
1989-06-01
影响因子：
1.1
通讯作者：
VIRTANEN, I
中科院分区：
文献类型：
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作者：
KARINIEMI, AL;VIRTANEN, I
我们使用三种单克隆细胞角蛋白抗体研究了良性表皮皮肤病和鲍文病中的角蛋白表达。在成人正常皮肤中，这些抗体仅与毛囊上皮 (PKK1)、基底角质形成细胞 (PKK2) 或毛囊间表皮中的基底上细胞 (KA5) 结合。此外，在胎儿表皮中，PKK1 抗体与基底角质形成细胞发生反应。在牛皮癣和扁平苔藓中，PKK2 抗体通过免疫染色清楚地显示了所有表皮细胞层。然而，用 KA5 抗体在两个病变中都显示出阴性基底细胞样层。在毛发红糠疹中，基底细胞层被 PKK2 抗体均匀染色，但只有上层细胞层的一些角质形成细胞显示荧光，而在慢性湿疹中，最低 3-4 层表皮细胞层呈反应性。 PKK1 抗体不会对所研究的任何良性增殖性皮肤病中的毛囊间角质形成细胞进行染色。在鲍文氏病中，使用所有抗体都观察到各个细胞之间具有不同强度的异质染色模式。我们的结果表明，慢性良性和恶性表皮疾病中角蛋白表达的不同变化可能反映了这些变化背后的机制。
We studied keratin expression in benign epidermal skin diseases, and in Bowen's disease by using three monoclonal cytokeratin antibodies. In adult normal skin, these antibodies bind only to the follicular epithelium (PKK1), the basal keratinocytes (PKK2), or the suprabasal cells in interfollicular epidermis (KA5). Additionally, in fetal epidermis, the PKK1 antibody reacts with basal keratinocytes. In psoriasis and lichen planus, the PKK2 antibody distinctly revealed all epidermal cell layers by immunostaining. However, a negative basal cell-like layer was revealed in both lesions with the KA5 antibody. In pityriasis rubra pilaris, the basal cell layer was uniformly stained with the PKK2 antibody, but only some keratinocytes in upper cell layers showed fluorescence and, in chronic eczema, the 3–4 lowest epidermal cell layers were reactive. The PKK1 antibody did not stain interfollicular keratinocytes in any of the benign proliferative skin diseases studied. In Bowen's disease, a heterogeneous staining pattern with varying intensity among individual cells was seen with all of the antibodies used. Our results suggest different changes in keratin expression in chronic benign and malignant epidermal diseases that may reflect the mechanisms behind these changes.