Identification of key events and regulatory networks in the formation process of primordial germ cell based on proteomics
Identification of key events and regulatory networks in the formation process of primordial germ cell based on proteomics
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DOI：
10.1002/jcp.30952
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发表时间：
2023-02
影响因子：
5.6
通讯作者：
Qisheng Zuo;Wei Gong;Zeling Yao;Qian Xia;Yani Zhang;Bichun Li
中科院分区：
文献类型：
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作者：
Qisheng Zuo;Wei Gong;Zeling Yao;Qian Xia;Yani Zhang;Bichun Li
目前，已有研究从转录水平分析了原始生殖细胞（Primordial germ cell，PGC）的形成机制，但从蛋白水平分析的研究较少，这使得对PGC形成机制的研究不够系统。在这里，我们筛选差异表达蛋白（DEP）调节PGC形成的无标记蛋白质组学与胚胎干细胞和PGC的一种新的采样策略。DEPs分析表明，PGC的形成涉及多个关键事件，如糖酵解向氧化磷酸化的转变、自噬的激活、DNA低甲基化保证了PGC的正常形成，除此之外，蛋白质泛素化也在PGC的形成中发挥了重要作用。重要的是，这些事件的进展归因于转录和翻译之间的不一致。有趣的是，MAPK、PPAR、Wnt和JAK信号通路不仅相互作用，而且还与不同的事件相互作用，参与PGC的形成，从而形成PGC调控网络。根据该调控网络，可以显著提高诱导系统中PGC形成的效率。研究结果表明，鸡PGC的形成是一个涉及多个事件和信号的复杂过程，为PGC研究的具体应用提供了技术支持。
Currently, studies have analyzed the formation mechanism of primordial germ cell (PGC) at the transcriptional level, but few at the protein level, which made the mechanism study of PGC formation not systematic. Here, we screened differential expression proteins (DEPs) regulated PGC formation by label‐free proteomics with a novel sampling strategy of embryonic stem cells and PGC. Analysis of DEPs showed that multiple key events were involved, such as the transition from glycolysis to oxidative phosphorylation, activation of autophagy, low DNA methylation ensured the normal formation of PGC, beyond that, protein ubiquitination also played an important role in PGC formation. Importantly, the progression of such events was attributed to the inconsistency between transcription and translation. Interestingly, MAPK, PPAR, Wnt, and JAK signaling pathways not only interact with each other but also interact with different events to participate in the formation of PGC, which formed the PGC regulatory network. According to the regulatory network, the efficiency of PGC formation in induction system can be significantly improved. In conclusion, our results indicate that chicken PGC formation is a complex process involving multiple events and signals, which provide technical support for the specific application in PGC research.