Glycosylation patterns are sexually dimorphic throughout development of the olfactory system in Manduca sexta
Glycosylation patterns are sexually dimorphic throughout development of the olfactory system in Manduca sexta
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DOI：
10.1002/cne.20178
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发表时间：
2004-08-09
影响因子：
2.5
通讯作者：
Tolbert, LP
中科院分区：
文献类型：
--
作者：
Gibson, NJ;Hildebrand, JG;Tolbert, LP
在雌雄蛾（Manduca sexta）中，成年嗅觉系统的发育依赖于触角嗅觉受体神经元、大脑前叶神经元和几种胶质细胞之间复杂的相互作用。作为表征可能在这些相互作用中发挥作用的分子的一种方法，我们使用凝集素筛选成体发育不同阶段的触角和触角叶。我们发现，每一种主要的神经细胞类型都有不同的凝集素标记模式。酶和其他处理对凝集素标记的影响使我们得出结论，主要的凝集素配体是：鞘糖脂和嗅觉受体神经元轴突上的o -连接、含聚焦的糖蛋白，触角叶神经元上的o -连接糖蛋白，以及初级嗅觉通路中所有类别的胶质细胞上的n -连接糖蛋白。小麦胚芽凝集素在发育过程中均匀地标记所有的嗅觉轴突，但在成年雄性中，标记仅限于对信息素敏感的嗅觉受体神经元。琥珀酰化的WGA更早地揭示了这些轴突类别的差异，因为肾小球是从原肾小球发育而来的。成年女性在针对普通肾小球和两性异型肾小球的轴突标记上表现出不太明显的差异。针对普通肾小球和性异形肾小球的受体轴突标记的差异可能与触角叶不同区域功能或连通性的差异有关。(C) 2004 Wiley-Liss, Inc。
In the moth Manduca sexta, development of the adult olfactory system depends, on complex interactions between olfactory receptor neurons in the antenna, antermal-lobe neurons in the brain, and several classes of glial cells. As one approach to characterizing molecules that may play roles in these interactions, we used lectins to screen antennae and antennal lobes at different stages of adult development. We find that each of the major neural cell types has a distinct pattern of labeling by lectins. Effects of enzymatic and other treatments on lectin labeling lead us to conclude that the predominant lectin ligands are: glycosphingolipids and an O-linked, fucose-containing glycoprotein on axons of olfactory receptor neurons, O-linked glycoproteins on antennal-lobe neurons, and N-linked glycoproteins on all classes of glial cells in the primary olfactory pathway. Wheat germ agglutinin labels all olfactory axons uniformly during much of development, but labeling becomes restricted to the pheromone-responsive olfactory receptor neurons in the adult male. Succinylated WGA reveals differences in these axon classes earlier, as glomerului develop from protoglomeruli. The adult female displays a less pronounced difference in labeling of axons targeting ordinary and sexually dimorphic glomeruli. Differences in labeling of receptor axons targeted to ordinary and sexually dimorphic glomeruli may be correlated with differences in function or connectivity in different regions of the antennal lobe. (C) 2004 Wiley-Liss, Inc.