Temporary expression of the TAF10 gene and its requirement for normal development of Arabidopsis thaliana
Temporary expression of the TAF10 gene and its requirement for normal development of Arabidopsis thaliana
复制标题
DOI：
10.1093/pcp/pcl048
复制
发表时间：
2007-01-01
影响因子：
4.9
通讯作者：
Izui, Katsura
中科院分区：
文献类型：
--
作者：
Tamada, Yosuke;Nakamori, Kazuki;Izui, Katsura
TAF10是TATA盒结合蛋白相关因子(TAFs)之一，与TBP共同构成TFIID。最初，大多数TAFs被认为是从一大群核心启动子准确启动转录所必需的。然而，最近的研究表明，在动物胚胎发育过程中，一些TAFs在有限的组织中表达，对组织的正常发育是必不可少的。他们被称为“选择性的”TAFs。然而，在植物中，人们对这些“选择性的”TAFs及其功能知之甚少。在这里，我们分离了拟南芥TAF10基因(AtTAF10)，这是一个与其他生物的TAF10基因密切相关的单基因。AtTAF10在侧根、莲座叶和大多数花器官的发育过程中瞬时表达。这种表达模式与拟南芥Rpb1的表达模式明显不同，拟南芥Rpb1编码RNA聚合酶II的一个组成部分，这表明atTAF10不仅在一般转录中发挥功能，而且还在部分基因的选择性表达中发挥作用。在atTAF10的敲除突变体中，我们观察到几种异常的表型参与了分生组织的活动和叶片的发育，这表明atTAF10参与了拟南芥的多效性，但具有特定的形态事件。这些结果清楚地表明TAF10在植物中是一种“选择性的”TAF，为研究TAFs在植物中的功能提供了新的视角。
TAF10 is one of the TATA box-binding protein (TBP)-associated factors (TAFs) which constitute a TFIID with a TBP. Initially most TAFs were thought to be necessary for accurate transcription initiation from a broad group of core promoters. However, it was recently revealed that several TAFs are expressed in limited tissues during animal embryogenesis, and are indispensable for normal development of the tissues. They are called 'selective' TAFs. In plants, however, little is known as to these 'selective' TAFs and their function. Here we isolated the Arabidopsis thaliana TAF10 gene (atTAF10), which is a single gene closely related to the TAF10 genes of other organisms. atTAF10 was expressed transiently during the development of several organs such as lateral roots, rosette leaves and most floral organs. Such an expression pattern was clearly distinct from that of Arabidopsis Rpb1, which encodes a component of RNA polymerase II, suggesting that atTAF10 functions in not only general transcription but also the selective expression of a subset of genes. In a knockdown mutant of atTAF10, we observed several abnormal phenotypes involved in meristem activity and leaf development, suggesting that atTAF10 is concerned in pleiotropic, but selected morphological events in Arabidopsis. These results clearly demonstrate that TAF10 is a 'selective' TAF in plants, providing a new insight into the function of TAFs in plants.