MOLECULAR-GENETIC ANALYSES OF THE TYROLEAN ICE MAN
MOLECULAR-GENETIC ANALYSES OF THE TYROLEAN ICE MAN
复制标题
DOI：
10.1126/science.8209259
复制
发表时间：
1994-06-17
期刊：
影响因子：
56.9
通讯作者：
PAABO, S
中科院分区：
文献类型：
--
作者：
HANDT, O;RICHARDS, M;PAABO, S
最近在蒂罗尔阿尔卑斯山发现了一具大约5000年前的木乃伊。从这个新石器时代晚期个体的组织样本中提取的DNA，被称为“冰人”，已经被提取和分析。在组织中存活的DNA分子数量大约为每克组织10个基因组当量，这意味着只能分析多拷贝序列。DNA的降解使得酶法扩增超过100至200个碱基的线粒体DNA片段变得困难。在两个不同的实验室从体内样本中独立确定了线粒体控制区高变片段的一个DNA序列。这一序列表明，冰人的线粒体类型符合当代欧洲人的遗传变异，并且与中欧和北方欧洲人的线粒体类型关系最为密切。
An approximately 5000-year-old mummified human body was recently found in the Tyrolean Alps. The DNA from tissue samples of this Late Neolithic individual, the so-called ''ice Man,'' has been extracted and analyzed. The number of DNA molecules surviving in the tissue was on the order of 10 genome equivalents per gram of tissue, which meant that only multi-copy sequences could be analyzed. The degradation of the DNA made the enzymatic amplification of mitochondrial DNA fragments of more than 100 to 200 base pal rs difficult. One DNA sequence of a hypervariable segment of the mitochondrial control region was determined independently in two different laboratories from internal samples of the body. This sequence showed that the mitochondrial type of the Ice Man fits into the genetic variation of contemporary Europeans and that it was most closely related to mitochondrial types determined from central and northern European populations.