Increased Expression of T-Box Transcription Factor Protein 21 (TBX21) in Skin Cutaneous Melanoma Predicts Better Prognosis: A Study Based on The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) Databases
Increased Expression of T-Box Transcription Factor Protein 21 (TBX21) in Skin Cutaneous Melanoma Predicts Better Prognosis: A Study Based on The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) Databases
复制标题
DOI：
10.12659/msm.923087
复制
发表时间：
2020-06-20
影响因子：
3.1
通讯作者：
Zhang, Longzhen
中科院分区：
文献类型：
--
作者：
Zhang, Xingying;Wen, Xin;Zhang, Longzhen
背景：T-box转录因子蛋白21（TBX 21）在免疫细胞和某些肿瘤细胞中表达。TBX 21基因缺陷可导致Th 1/Th 2失衡，与肿瘤的发生密切相关。材料/方法：从癌症基因组图谱（TCGA）和基因型-组织表达（GTEx）数据库中下载TBX 21基因的表达和临床资料。采用Wilcoxon符号秩检验和Logistic回归分析TBX 21表达与性别、分期等临床参数的关系，采用考克斯回归和Kaplan-Meier法分析SKCM患者临床病理特征与总生存期的相关性。采用基因集富集分析（GSEA）和蛋白质相互作用（PPI）分析TBX 21在SKCM中的作用机制。根据Kaplan-Meier生存分析，TBX 21低表达的SKCM患者的预后可能比TBX 21高表达的患者更差。考克斯分析也得出同样的结论：TBX 21是一个独立的预后指标。GSEA结果显示TBX 21中高表达的表型不同程度地富集了多种信号通路。PPI网络显示与TBX 21密切相关的前10个蛋白。结论：TBX 21的表达与SKCM患者的预后密切相关，并参与多种影响肿瘤发生发展的免疫学途径。
Background: T-box transcription factor protein 21 (TBX21) is expressed in immune cells and some tumor cells. Defects in TBX21 gene can cause Th1/Th2 imbalance, which is closely related to tumorigenesis. The expression and clinical value of TBX21 in skin cutaneous melanoma (SKCM) are not clear.Material/Methods: RNA-Seq expression and clinical information were downloaded from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases. Wilcoxon signed-rank test and logistic regression were used to explore the relationship between TBX21 expression and clinical parameters such as gender, stage, etc. The correlation between clinicopathological characteristics and overall survival of SKCM patients was estimated by Cox regression and the Kaplan-Meier method. Gene set enrichment analysis (GSEA) and protein-protein interaction (PPI) were conducted to analyze the potential mechanism of TBX21 in the progression of SKCM.Results: Compared with normal samples, TBX21 was significantly upregulated in SKCM tissues. SKCM patients with lower TBX21 expression might have a worse prognosis than those with higher TBX21 expression according to Kaplan-Meier survival analysis. Cox analysis also reached the same conclusion: TBX21 was an independent prognostic indicator. GSEA showed that the highly expressed phenotypes in TBX21 were enriched to varying degrees with various signaling pathways. PPI network showed the top 10 proteins that were closely related to TBX21.Conclusions: TBX21 expression was significantly correlated with the prognosis of SKCM patients and was found to be involved in a great many immunological pathways that affect the occurrence and development of tumors.