mRNA transcript distribution bias between Borrelia burgdorferi bacteria and their outer membrane vesicles.
mRNA transcript distribution bias between Borrelia burgdorferi bacteria and their outer membrane vesicles.
复制标题
伯氏疏螺旋体细菌与其外膜囊泡之间的 mRNA 转录本分布偏差。
DOI：
10.1093/femsle/fny135
复制
发表时间：
2018
影响因子：
2.1
通讯作者：
Wang,Kai
中科院分区：
文献类型：
--
作者：
Malge,Anjali;Ghai,Vikas;Reddy,PangaJaipal;Baxter,David;Kim,Taek-Kyun;Moritz,RobertL;Wang,Kai
伯氏疏螺旋体（Borrelia burgdorferi）是螺旋体门的一种细菌，是莱姆病的病原体。细菌，包括B. burgdorferi，释放球形外膜囊泡（OMV），已知其携带包括代谢物、核酸和蛋白质的分泌产物。在此，我们提供了第一个比较转录组学分析的囊泡释放从B。我们从细菌细胞及其OMV中鉴定了总共101200个独特的转录物，其具有至少一个映射的读段。我们比较了细菌细胞和其OMV之间的转录物谱，发现基于转录物来源的偏向性分布，即质粒编码的转录物更可能在OMV中富集。我们通过qPCR验证了一些转录物的分布。这一分析提供了第一个证据，表明一些B。Burgdorferitranscripts优先包装在OMV中，这进一步表明细菌可能使用其OMV进行细菌-细菌或细菌-宿主通信。这份报告还表明，在莱姆病的早期和疾病后综合征的发展中可能涉及疏螺旋体衍生的OMV。
Borrelia burgdorferi, a bacterium in the spirochete phylum, is the causative agent of Lyme disease.Borrelia burgdorferihas a linear chromosome with a number of circular and linear plasmids. Bacteria, includingB. burgdorferi, release spherical outer membrane vesicles (OMVs) that are known to carry secretory products including metabolites, nucleic acids and proteins. Herein, we provide the first comparative transcriptomic analysis of the vesicles released fromB. burgdorferi.We identified a total of ∼1200 unique transcripts with at least one mapped read from the bacterial cell and its OMVs. We compared the spectrum of transcripts between bacterial cell and its OMVs, and found a biased distribution based on the source of transcripts, i.e. plasmid-encoded transcripts are more likely to be enriched in the OMVs. We validated the distribution for some of the transcripts by qPCR. This analysis provides the first evidence that some of theB. burgdorferitranscripts are preferentially packaged in OMV, which further suggest that the bacteria might use its OMVs for bacteria–bacteria or bacteria–host communications. This report also suggests a possible involvement ofBorrelia-derived OMVs in the development of Lyme disease in both early and post disease syndromes.