Induction of interleukin-6 in axotomized sensory neurons
Induction of interleukin-6 in axotomized sensory neurons
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DOI：
10.1523/jneurosci.15-07-05130.1995
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发表时间：
1995-07
期刊：
影响因子：
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通讯作者：
P. Murphy;J. Grondin;M. Altares;P. Richardson
中科院分区：
文献类型：
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作者：
P. Murphy;J. Grondin;M. Altares;P. Richardson
分析来自大鼠背根神经节的RNA以寻找由神经损伤在背根神经节中诱导的潜在有益的细胞因子。通过逆转录，PCR和Southern印迹，白细胞介素-6 mRNA的检测在发展过程中，但没有在正常成人背根神经节，出现在1 d内的坐骨神经横断，最大增加后2和4 d，并在1周内低于检测阈值。通过RNA酶保护试验，白细胞介素-6 mRNA始终存在于RNA从大鼠背根神经节切断后4天，但不是在对照背根神经节。白细胞介素-6生物活性也存在于神经损伤后的背根神经节。原位杂交结果显示，白细胞介素-6 mRNA定位于大、中型轴突切断的神经元内.总之，一些感觉神经元对轴突切断的反应是白细胞介素-6的合成迅速增加。坐骨神经损伤还诱导背根神经节中白细胞介素-1 β和肿瘤坏死因子-α的mRNA。白细胞介素-1 β和肿瘤坏死因子-α mRNA的诱导比白细胞介素-6 mRNA的诱导更晚且更持续。这两种细胞因子的细胞来源尚未确定。
RNA from rat dorsal root ganglia was analyzed in search of potentially beneficial cytokines that are induced in dorsal root ganglia by nerve injury. By reverse transcription, the PCR, and Southern blotting, interleukin-6 mRNA was detected during development but not in normal adult dorsal root ganglia, reappeared within 1 d of sciatic nerve transection, was maximally increased after 2 and 4 d, and decreased below the threshold of detection within 1 week. By RNase protection assay, interleukin-6 mRNA was consistently present in RNA from dorsal root ganglia removed from rats 4 d following transection but not in control dorsal root ganglia. Interleukin-6 bioactivity was also present in dorsal root ganglia following nerve injury. By in situ hybridization, interleukin-6 mRNA was localized within large and medium- sized axotomized neurons. In summary, some sensory neurons respond to axotomy with a brisk transient increase in synthesis of interleukin-6. Injury to the sciatic nerve also induced mRNAs for interleukin-1 beta and tumor necrosis factor-alpha in dorsal root ganglia. The inductions of interleukin-1 beta and tumor necrosis factor-alpha mRNAs were later and more sustained than that of interleukin-6 mRNA. The cellular sources of these two cytokines have not been defined.