Surface modification of Mitoxantrone-loaded PLGA nanospheres with chitosan
Surface modification of Mitoxantrone-loaded PLGA nanospheres with chitosan
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DOI：
10.1016/j.colsurfb.2009.05.020
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发表时间：
2009-10-15
影响因子：
5.8
通讯作者：
Wang, Yinsong
中科院分区：
文献类型：
--
作者：
Chen, Hongli;Yang, Wenzhi;Wang, Yinsong
本研究的目的是制备壳聚糖(CS)表面修饰的聚乳酸-羟基乙酸(PLGA)纳米球。采用溶剂挥发法制备了米托蒽醌(MTO)PLGA纳米球。采用两种策略(吸附和共价结合)对PLGA纳米球表面进行CS修饰。用激光光散射法对粒径为248.4+/-21 nm的聚乳酸纳米球进行了表征，扫描电子显微镜显示其为球形，包封率为84.1+/-3.4%。未改性纳米球的Zeta电位为-21.21+/-2.13 mV。修饰后的纳米球的Zeta电位为正，说明修饰后的纳米球表面存在CS。用X射线光电子能谱(XPS)和傅立叶变换红外光谱(FT-IR)对改性后的纳米球进行了表面化学表征。FT-IR谱在3420 cm(-1)和1570 cm(-1)处出现峰，XPS谱显示纳米球表面的N1s(原子轨道是氮的)区域，对应于CS的伯胺。体外药物释放实验表明，壳聚糖修饰纳米球与未修饰纳米球相比具有药物释放时间延长、突释减少等优点，采用共价结合的方法修饰后的纳米球可以达到相对恒定的释药动力学。这些数据表明CS修饰的PLGA纳米球作为抗癌药物载体具有很高的潜力。皇冠版权所有(C)2009由爱思唯尔出版。保留所有权利。
The purpose of this research was to develop polylactic-co-glycolic acid (PLGA) nanospheres surface modified with chitosan (CS). Mitoxantrone- (MTO-) loaded PLGA nanospheres were prepared by a solvent evaporation technique. The PLGA nanospheres surface was modified with CS by two strategies (adsorption and covalent binding). PLGA nanospheres of 248.4 +/- 21.0 nm in diameter characterized by the laser light scattering technique, scanning electron microscopy (SEM) are spherical and its drug encapsulation efficiency is 84.1 +/- 3.4%. Zeta potential of unmodified nanospheres was measured to be negative -21.21 +/- 2.13 mV. The positive zeta potential of modified nanospheres reveals the presence of CS on the surface of the modified nanospheres. Modified nanospheres were characterized for surface chemistry by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FT-IR). FT-IR spectra exhibited peaks at 3420 cm(-1) and 1570 cm(-1), XPS spectra shows the N 1s (atomic orbital is of nitrogen) region of the surface of the nanospheres, corresponding to the primary amide of CS. In vitro drug release demonstrated that CS-modified nanospheres have many advantages such as prolonged drug release property and decreased the burst release over the unmodified nanospheres, and the modified nanospheres by covalent binding method could achieve the release kinetics of a relatively constant release. These data demonstrate high potential of CS-modified PLGA nanospheres for the anticancer drug carrier. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.