Automated immunoturbidimetric analysis of six plasma apolipoproteins: Correlation with radial immunodiffusion assays
Automated immunoturbidimetric analysis of six plasma apolipoproteins: Correlation with radial immunodiffusion assays
复制标题
六种血浆载脂蛋白的自动免疫比浊分析：与径向免疫扩散测定的相关性
DOI：
10.1002/jcla.1860050204
复制
发表时间：
1991
影响因子：
2.7
通讯作者：
H. Okabe
中科院分区：
文献类型：
--
作者：
Teruaki Ikeda;Yohko Shibuya;U. Senba;H. Sugiuchi;Shukuro Araki;Y. Uji;H. Okabe
我们使用自动离散生化分析仪和市售抗血清通过比浊法测定了6种载脂蛋白（apo AI、AII、B、CII、CIII和E）。并与单向免疫扩散法进行了比较。自动浊度法测定血清载脂蛋白的线性好于单向免疫扩散法。浊度法测定的线性范围为：AI为2.5 G/L，AII为1.0 G/L，B为4.5 G/L，CII为0.12 G/L，CIII为0.3 G/L，E为0.12 G/L。存在高浓度胆红素（高达0.15 G/L）和血红蛋白（高达50 G/L）干扰载脂蛋白测定。免疫比浊法和单向免疫扩散法（SRID）的比较显示，载脂蛋白AI、AII、B、CII、CIII和E的相关系数分别为0.963、0.896、0.846、0.936、0.972和0.937。
We measured six apolipoproteins (apo AI, AII, B, CII, CIII, and E) by turbidimetric method using an automatic discrete biochemical analyzer and commercially available antisera. The turbidimetric method was compared with the single radial immunodiffusion method. Linearity for serum apolipoprotein assay by the automated turbidimetric method was better than by the single immunodiffusion method. The linearity by the turbidimetric method was 2.5 G/L for AI, 1.0 G/L for AII, 4.5 G/L for B, 0.12 G/L for CII, 0.3 G/L for CIII, and 0.12 G/L for E. The presence of high concentrations of bilirubin (up to 0.15 G/L) and hemoglobin (up to 50 G/L) interfered with apolipoprotein measurement. Comparison of the immunoturbidimetric and the single radial immunodiffusion (SRID) methods showed excellent coefficients of correlation, r = 0.963, 0.896, 0.846, 0.936, 0.972, and 0.937 for apo AI, AII, B, CII, CIII, and E, respectively.