The performance of bone marrow mesenchymal stem cell--implant complexes prepared by cell sheet engineering techniques.
The performance of bone marrow mesenchymal stem cell--implant complexes prepared by cell sheet engineering techniques.
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DOI：
10.1016/j.biomaterials.2010.01.036
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发表时间：
2010-04
期刊：
影响因子：
14
通讯作者：
W. Zhou;Chun Han;Yingliang Song;Xingrong Yan;Dehua Li;Z. Chai;Zhi-hong Feng;Yan Dong;Liwen Li;Xin Xie;Fulin Chen;Yimin Zhao
中科院分区：
文献类型：
--
作者：
W. Zhou;Chun Han;Yingliang Song;Xingrong Yan;Dehua Li;Z. Chai;Zhi-hong Feng;Yan Dong;Liwen Li;Xin Xie;Fulin Chen;Yimin Zhao
本研究探讨了由多层兔骨髓间充质干细胞（MSC）组成的细胞片可以与两种植入物（表面改性钛和氧化锆）组装以构建MSC植入物的假设。从培养瓶中收获MSC片，包裹在植入物周围以构建复合物，然后在成骨培养基中培养。分层的细胞片与植入物整合良好，并保持活力，在培养后长达四周的植入物表面上可见小的矿化结节。植入物上的细胞经历了经典的体外成骨分化，伴随着碱性磷酸酶活性和骨和血管相关蛋白表达的升高。在体内，将两种细胞片-植入物复合物移植到SCID小鼠皮下并培养8周。对于MSC片状钛植入物复合体，组织学检查显示，在植入物周围形成的新骨组织遵循主要的软骨内途径，显示出天然骨的组织学标记;然而，对于MSC片氧化锆植入物复合体，膜内骨化似乎发生在氧化锆植入物的表面上，如典型的骨细胞包埋在致密基质中并伴有微血管和骨髓腔所观察到的。这些研究结果表明，具有成骨和血管化能力的MSC-植入物可以使用细胞片工程技术结合常规植入物材料，这提供了一种新的技术来修改植入物表面。
This study investigated the hypothesis that cell sheets composed of multilayered rabbit bone marrow derived mesenchymal stem cells (MSC) could be assembled with two kinds of implants (surface-modified titanium and zirconia) for the construction of a MSC-implant. The MSC sheets were harvested from culture flasks, wrapped around implants to construct the complexes, and then cultured in osteogenic medium. The layered cell sheets integrated well with implants and remained viable, with small mineralized nodules visible on the implant surfaces for up to four weeks after culture. Cells on the implants underwent classical in vitro osteogenic differentiation with an associated elevation of alkaline phosphatase activity and bone- and vascular-related protein expression. In vivo, two kinds of cell sheet–implant complexes were transplanted under the skin of SCID mice and cultured for eight weeks. For the MSC sheet titanium implant complex, histological examination revealed that new bone tissue that formed around implants followed a predominantly endochondral pathway, exhibiting histological markers of native bone; for the MSC sheet zirconia implant complex, however, intramembranous ossification appeared to occur on the surface of the zirconia implant, as observed with typical osteocytes embedded in dense matrix and accompanied by both microvessels and marrow cavities. These findings demonstrate that MSC-implants possessing osteogenic and vascularization abilities can be produced using cell sheet engineering techniques in conjunction with routine implant materials, which provide a novel technology to modify the implant surface.