PHOSPHOLIPID-COMPOSITION AND SURFACE-ACTIVE PROPERTIES OF TRACHEOBRONCHIAL SECRETIONS FROM PATIENTS WITH CYSTIC-FIBROSIS AND CHRONIC OBSTRUCTIVE PULMONARY-DISEASES
PHOSPHOLIPID-COMPOSITION AND SURFACE-ACTIVE PROPERTIES OF TRACHEOBRONCHIAL SECRETIONS FROM PATIENTS WITH CYSTIC-FIBROSIS AND CHRONIC OBSTRUCTIVE PULMONARY-DISEASES
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DOI：
10.1002/ppul.1950130107
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发表时间：
1992-05-01
影响因子：
3.1
通讯作者：
PUCHELLE, E
中科院分区：
文献类型：
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作者：
GIROD, S;GALABERT, C;PUCHELLE, E
在气管支气管分泌物的各种成分中，脂质，特别是磷脂已被证明会影响囊性纤维化患者气道分泌物的流变学性质。我们研究了从患有囊性纤维化（CF）和其他慢性阻塞性肺疾病（COPD）的患者收集的气管支气管分泌物的磷脂组成，并分析了通过测量接触角评估的气管支气管分泌物的磷脂分布和润湿性之间的可能关系。CF组气管支气管分泌物的总磷脂含量和接触角均显著高于COPD组（P < 0.01），但二者之间无显著相关性。不同磷脂亚类的浓度根据分泌物的来源没有均匀地改变。与COPD分泌物相比，CF分泌物的特征在于硬化组分如鞘磷脂和磷脂酰丝氨酸/磷脂酰肌醇显著（P < 0.001）增加，表面活性组分如磷脂酰胆碱和磷脂酰甘油（PG）显著（P < 0.001）减少（P < 0.001）。在两组中，表面活性磷脂组分PG与气管支气管分泌物的接触角呈负相关。这些结果表明，CF分泌物中PG含量的减少可能是导致其对呼吸道粘膜的粘附性增加的一个因素，因此，囊性纤维化中粘液淤滞和支气管阻塞的严重程度。
Among the various components of tracheobronchial secretions, lipids and particularly phospholipids have been shown to influence rheological properties of airway secretions in patients with cystic fibrosis. We studied the phospholipid composition of tracheobronchial secretions, collected from patients suffering from cystic fibrosis (CF) and other chronic obstructive pulmonary diseases (COPD), and we analyzed the possible relationship between the phospholipid profile and the wettability of tracheobronchial secretions evaluated by the measurement of contact angle. Although total phospholipid content and contact angle of tracheobronchial secretions were significantly increased (P < 0.01) in CF compared to COPD, no significant relationship existed between these two parameters. The concentrations of the different phospholipid subclasses were not homogeneously modified according to the origin of the secretions. Compared to COPD secretions, the CF secretions were characterized by a significant (P < 0.001) increase in rigidifying fractions such as sphingomyelin and phosphatidylserine/phosphatidylinositol and a significant (P < 0.001) decrease in surface-active fractions, such as phosphatidylcholine and phosphatidylglycerol (PG) (P < 0.001). In the two groups, the surface-active phospholipid fraction, PG, was negatively correlated to the contact angle of tracheobronchial secretions. These results suggest that a decrease in PG content in CF secretions may be one factor responsible for an increase in their adhesivity to the respiratory mucosa, and, consequently, for mucus stasis and severity of bronchial obstruction in cystic fibrosis.