Isolation and Characterization of Murine Multipotent Lung Stem Cells
Isolation and Characterization of Murine Multipotent Lung Stem Cells
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DOI：
10.1089/scd.2009.0287
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发表时间：
2010-04-01
影响因子：
4
通讯作者：
Yamaya, Mutsuo
中科院分区：
文献类型：
--
作者：
Hegab, Ahmed E.;Kubo, Hiroshi;Yamaya, Mutsuo
肺损伤后的自我修复能力是众所周知的，但参与肺再生的细胞类型仍不明确。本研究的目的是从成年小鼠肺中分离和鉴定常驻祖细胞/干细胞。我们报告了具有Sca 1（+）/CD 45（-）/CD 31（-）表型的常驻干细胞的分离和表征。它们的免疫表型和分化潜能与以前描述的其他肺干细胞不同。这些细胞在培养中进行了广泛的自我更新，并能在体外分化为内皮和肺上皮（肺泡I型，II型和克拉拉）细胞。它们表现出一定的间充质分化能力，但没有神经分化能力。将这些细胞转移到肺损伤的小鼠模型中显着提高了存活率并最大限度地减少了肺损伤。这些细胞可能为研究肺干细胞和评估肺部疾病的新治疗方法提供有用的工具。
The capacity of the lung to repair itself after injury is well known, but the cell types involved in lung regeneration remain undefined. The aim of this study was to isolate and characterize resident progenitor/stem cells from adult mouse lung. We report the isolation and characterization of resident stem cells that have a Sca1(+)/CD45(-)/CD31(-) phenotype. Their immunophenotype and differentiative potentiality were distinct from that of other previously described lung stem cells. These cells underwent extensive self-renewal in culture and could differentiate into endothelial and lung epithelial (alveolar type I, II, and Clara) cells in vitro. They have exhibited some mesenchymal but no neural differentiation ability. Transfer of these cells into mouse models of lung injury significantly improved survival and minimized lung destruction. These cells may provide useful tools for the study of lung stem cells and the assessment of new therapeutic approaches for lung diseases.