Wound healing after applying a gelatin-based hydrogel sheet to resected rat submandibular gland.
Wound healing after applying a gelatin-based hydrogel sheet to resected rat submandibular gland.
复制标题
将明胶水凝胶片应用于切除的大鼠颌下腺后伤口愈合。
DOI：
10.2334/josnusd.19-0244
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发表时间：
2020
影响因子：
1.9
通讯作者：
M. Tonogi
中科院分区：
文献类型：
--
作者：
Yusuke Miyake;O. Shimizu;H. Shiratsuchi;Takaaki Tamagawa;M. Tonogi
本研究旨在建立生理活性物质对唾液腺腺泡细胞萎缩和再生影响的实验模型。8周龄Wistar大鼠（n = 24）在植入明胶基水凝胶片后的第3、5、7、10、14和21天，在颌下腺切除伤口（直径3 mm）进行组织化学检查。结果显示，到第10天，这张纸几乎消失了。切除伤口周围的区域分为正常、萎缩和坏死。在萎缩区，切除后腺泡细胞萎缩，第7天腺泡细胞较少。第5-7天，呈条状和粒状的导管呈导管状结构。第10天，通过周期性酸-希夫染色证实新形成的腺泡细胞增多，此后成熟细胞数量增加。在坏死区域，腺泡和导管细胞被破坏，在第3天出现分散的去核腺泡细胞和导管样结构；第10天观察到新形成的腺泡细胞。因此，实验模型显示了颌下腺的萎缩和再生，并能够分析植入片中含有的生理活性物质的持续释放的影响。
The present study was done to develop a useful experimental model for analysis of the effects of physiologically active substances on atrophy and regeneration of salivary gland acinar cells. Resection wounds (diameter, 3 mm) were made in the submandibular glands of 8-week-old Wistar rats (n = 24) for histochemical examination on Days 3, 5, 7, 10, 14, and 21 after implantation of a gelatin-based hydrogel sheet. The results showed that the sheet had nearly disappeared by Day 10. Regions around the resection wounds were classified as normal, atrophic, or necrotic. In atrophic regions, acinar cells atrophied after resection, and few acinar cells were observed on Day 7. On Days 5-7, striated and granular ducts resembled duct-like structures. On Day 10, newly formed acinar cells were confirmed by increased periodic acid-Schiff staining, and a greater number of mature cells was present thereafter. In necrotic regions, acinar and ductal cells were destroyed, and scattered enucleated acinar cells and duct-like structures were present, on Day 3; newly formed acinar cells were observed on Day 10. Thus, the experimental model demonstrated atrophy and regeneration of the submandibular gland and enabled analysis of the effects of sustained release of physiologically active substances contained within an implanted sheet.