Cerebellar cell surface antigens of mouse brain.
Cerebellar cell surface antigens of mouse brain.
复制标题
小鼠脑的小脑细胞表面抗原。
DOI：
--
复制
发表时间：
1975
影响因子：
11.1
通讯作者：
N. Seeds
中科院分区：
文献类型：
--
作者：
N. Seeds
来自6至8天龄小鼠小脑的重新聚集的细胞已被用于在兔中产生抗体。这些抗体与小脑细胞表面成分的相互作用进行了评估的51铬标记的小脑细胞培养物的细胞毒性和间接免疫荧光。对来自小鼠其他组织、不同年龄小鼠和突变小鼠的脑区、小脑和其他动物种属的细胞以及神经系统来源的几种克隆细胞系的抗原相对量进行了定量比较。在与补体和51Cr标记的小脑靶细胞孵育之前，用越来越多的解离细胞或颗粒组织量吸附固定的阈下浓度的抗血清。小鼠胸腺、脾脏、肝脏和心脏组织具有可忽略的吸附能力，而肾脏和精子具有一定的吸附能力。在检查的大脑区域中，只有小脑去除了所有的免疫荧光和细胞毒性活性，而其他区域去除了不到90%，这表明某些细胞类型上的小脑特异性抗原的可能性。只有小鼠和大鼠小脑给予可测量的吸附，这种能力随着年龄的增长而下降。虽然小脑突变体（交错，韦弗，和神经）具有类似的吸附能力，胶质瘤和神经母细胞瘤克隆细胞系不同的可测量的吸附，只有小鼠神经母细胞瘤克隆显示显着吸附的抗血清。
Reaggregated cells from 6- to 8-day-old mouse cerebella have been used to raise antibodies in rabbits. The interaction of these antibodies with cerebellar cell surface components was assessed by cytotoxicity of 51Cr-labeled cerebellar cell cultures and indirect immunofluorescence. A quantitative comparison of the relative amount of antigen on cells from other mouse tissues, brain regions, cerebella of various aged mice and mutant mice, and other animal species, as well as several clonal cell lines of nervous system origin, was made. A fixed subthreshold concentration of antiserum was adsorbed with increasing numbers of dissociated cells or amounts of particulate tissue prior to incubation with complement and 51Cr-labeled cerebellar target cells. Mouse thymus, spleen, liver, and heart tissue possess negligible adsorbing capacity, whereas kidney and sperm gave some adsorption. Of the brain regions examined, only cerebellum removed all immunofluorescence and cytotoxic activity, whereas other regions removed less than 90%, suggesting the possibility of cerebellar specific antigens on certain cell types. Only mouse and rat cerebellum gave measurable adsorptions, and this capacity decreased with increasing age. Although cerebellar mutants (stagger, weaver, and nervous) possessed similar adsorptive capacity, glioma and neuroblastoma clonal cell lines differed measurably in their adsorption; only the mouse neuroblastoma clones displayed significant adsorption of the antiserum.