Twin studies on the effect of genetic factors on serum agalactosyl immunoglobulin G levels
Twin studies on the effect of genetic factors on serum agalactosyl immunoglobulin G levels
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DOI：
10.3892/br.2014.216
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发表时间：
2014-03-01
影响因子：
2.3
通讯作者：
Miyoshi, Eiji
中科院分区：
文献类型：
--
作者：
Azuma, Kanako;Shinzaki, Shinichiro;Miyoshi, Eiji
发现缺乏末端半乳糖的免疫球蛋白G（IgG）（称为无半乳糖基IgG）的水平在慢性炎性疾病如类风湿性关节炎和炎性肠病（IBD）中增加，特别是在克罗恩病中，其被认为具有遗传成分。IgG的这种寡糖修饰主要受糖基因表达的调节;然而，遗传因素与IgG糖基化变化之间的关联尚未完全阐明。本研究的目的是评估遗传学在这一过程中的作用，通过比较同卵双胞胎和异卵双胞胎同时接受体检的成员之间的血清无半乳糖基IgG水平。采用高效液相色谱法测定血清无半乳糖基IgG水平。还测量了血液学和生化标志物，包括γ-谷氨酰转肽酶（γ GTP）、丙氨酸氨基转移酶（ALT）和白色血细胞（WBC）计数。尽管血清GTP水平（以及较小程度上ALT和WBC水平）在同卵双胞胎中表现出相关性，但未发现无半乳糖基IgG水平在任一类型双胞胎成员之间相关。因此，遗传因素在确定血清无半乳糖基IgG水平中的作用可能不如环境因素或炎症性疾病发作的影响显著。
The level of immunoglobulin G (IgG) lacking the terminal galactose, referred to as agalactosyl IgG, was found to be increased in chronic inflammatory diseases, such as rheumatoid arthritis and inflammatory bowel disease (IBD), particularly in Crohn's disease, which is suggested to have a genetic component. This oligosaccharide modification of IgG is mainly regulated by the expression of glyco-genes; however, the association between genetic factors and changes in the IgG glycosylation has not been fully elucidated. The aim of the present study was to assess the role of genetics in this process by comparing the serum agalactosyl IgG levels between members of monozygotic and dizygotic twin pairs who underwent medical check-ups at the same time. The serum agalactosyl IgG level was assayed using high-performance liquid chromatography. Hematological and biochemical markers, including gamma-glutamyltranspeptidase (gamma GTP), alanine aminotransferase (ALT) and white blood cell (WBC) count, were also measured. Although the serum.GTP levels (and, to a lesser extent, ALT and WBC levels) exhibited a correlation within monozygotic twin pairs, agalactosyl IgG levels were not found to be correlated between members of either type of twin pairs. Thus, the role of genetic factors in determining serum agalactosyl IgG levels may be less significant compared to the effect of environmental factors or the onset of inflammatory disease.