Hydroxyurea-induced synchronisation of bloodstream stage Trypanosoma brucei
Hydroxyurea-induced synchronisation of bloodstream stage Trypanosoma brucei
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DOI：
10.1016/j.molbiopara.2008.12.008
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发表时间：
2009-04-01
影响因子：
1.5
通讯作者：
Hammarton, Tansy C.
中科院分区：
文献类型：
--
作者：
Forsythe, Glynn R.;McCulloch, Richard;Hammarton, Tansy C.
布氏锥虫细胞周期的同步化多年来一直难以实现。最近的一份报告表明，同步化的前环形式的细胞是可能的治疗后，与羟基脲。在这里，这项工作被扩展到疾病相关的，锥虫感染的血流阶段锥虫。用10 μ g ml（-1）羟基脲处理体外生长的血流期Lister 427布氏T细胞6小时，导致S期细胞富集。去除药物后，细胞均匀地进行一轮细胞周期，提供了急需的工具来富集特定的细胞周期阶段，其方式类似于前环形式T的羟基脲处理。布鲁塞。(C)2009爱思唯尔有限公司版权所有。
Synchronisation of the Trypanosoma brucei cell cycle proved elusive for many years. A recent report demonstrated that synchronisation of procyclic form cells was possible following treatment with hydroxyurea. Here, that work is extended to the disease-relevant, mammalian-infective bloodstream stage trypanosome. Treatment of bloodstream stage Lister 427 T brucei cells growing in vitro with 10 mu g ml(-1) hydroxyurea for 6 h led to an enrichment of cells in S phase. Following removal of the drug, cells proceeded uniformly through one round of the cell cycle, providing a much needed tool to enrich for specific cell cycle stages, in a manner similar to hydroxyurea treatment of procyclic form T. brucei. (C) 2009 Elsevier B.V. All rights reserved.