Biologically Triggered Delivery of EGF from Polymer Fiber Patches.
Biologically Triggered Delivery of EGF from Polymer Fiber Patches.
复制标题
生物触发从聚合物纤维贴片输送 EGF。
DOI：
10.1021/acsmacrolett.7b00212
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发表时间：
2017
影响因子：
7.015
通讯作者：
Pokorski,JonathanK
中科院分区：
文献类型：
--
作者：
Kim,Si-Eun;Lee,ParkerW;Pokorski,JonathanK
伤口愈合通过伤口部位内生长因子的复杂空间和时间调节来调节。再生医学试图产生能够模拟这种环境的材料，用于慢性或创伤性伤口的愈合。在此，我们报告了表皮生长因子（EGF）从共挤出聚合物纤维贴片，这是由伤口愈合的自然生物级联触发的程序性释放。将含有基质金属蛋白酶（MMP）切割位点的基因工程EGF共价结合到通过多层熔融共挤出制造的非织造聚（ε-己内酯）（PCL）纤维垫上。基因修饰的EGF在生物触发剂MMP-9的存在下显示出快速释放，而对照蛋白质显示出可忽略的释放。在MMP-9存在的情况下，生物响应纤维垫显着增强了人类角质形成细胞的增殖和迁移。这项研究描述了一种关键的伤口愈合生长因子的释放，这是由愈合过程中固有的生物学触发的。
Wound healing is modulated by complex spatial and temporal regulation of growth factors within a wound site. Regenerative medicine seeks to generate materials that can mimic this environment for the healing of chronic or traumatic wounds. Herein, we report a programmed release of epidermal growth factor (EGF) from coextruded polymer fiber patches, which is triggered by the natural biological cascade of wound healing. Genetically engineered EGF containing a matrix metalloproteinase (MMP) cleavage site was covalently conjugated to a nonwoven poly(ε-caprolactone) (PCL) fiber mat fabricated by multilayered melt coextrusion. The genetically modified EGF showed rapid release in the presence of a biological trigger, MMP-9, while a control protein showed negligible release. The biologically responsive fiber mat dramatically enhanced proliferation and migration of human keratinocytes in the presence of MMP-9. This study describes the release of a critical wound-healing growth factor as triggered by the biology inherent in the healing process.