Fully desktop fabricated flexible graphene electrocorticography (ECoG) arrays
Fully desktop fabricated flexible graphene electrocorticography (ECoG) arrays
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DOI：
10.1088/1741-2552/acae08
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发表时间：
2023-02-01
影响因子：
4
通讯作者：
Kodandaramaiah, Suhasa B.
中科院分区：
文献类型：
--
作者：
Hu, Jia;Hossain, Ridwan Fayaz;Kodandaramaiah, Suhasa B.
目的：柔性皮质电图（ECoG）电极阵列符合皮质表面并记录多个大脑区域的表面场电位，为分布式大脑区域中发生的计算如何介导行为提供了独特的见解。需要专门的微加工方法来生产具有高密度电极阵列的柔性 ECoG 设备。然而，这些制造方法对于无法使用洁净室制造设备的科学家来说具有挑战性。结果：在这里，我们展示了一个完全桌面制造的柔性石墨烯 ECoG 阵列。首先，我们通过昔兰尼中石墨烯的液体剥离合成了一种稳定的导电墨水。接下来，我们建立了一种模板印刷工艺，通过柔性聚酰亚胺基板上的激光切割模板对石墨烯墨水进行图案化。台式测试表明，石墨烯电极具有约1.1 x 10(3) S cm(-1)的良好电导率、在静态弯曲下保持电连接的灵活性以及在15 d加速腐蚀测试中的电化学稳定性。长期植入的石墨烯 ECoG 装置可在长达 180 天的时间内保持完整功能，1 kHz 时的平均体内阻抗为 24.72 +/- 95.23 k Ω。 ECoG 设备可以测量小鼠在清醒和麻醉状态下的自发表面场电位以及感官刺激诱发的反应。意义：模板印刷制造工艺可用于使用常用实验室设备在 24 小时内创建具有定制电极布局的石墨烯 ECoG 设备。
Objective: Flexible Electrocorticography (ECoG) electrode arrays that conform to the cortical surface and record surface field potentials from multiple brain regions provide unique insights into how computations occurring in distributed brain regions mediate behavior. Specialized microfabrication methods are required to produce flexible ECoG devices with high-density electrode arrays. However, these fabrication methods are challenging for scientists without access to cleanroom fabrication equipment. Results: Here we present a fully desktop fabricated flexible graphene ECoG array. First, we synthesized a stable, conductive ink via liquid exfoliation of Graphene in Cyrene. Next, we established a stencil-printing process for patterning the graphene ink via laser-cut stencils on flexible polyimide substrates. Benchtop tests indicate that the graphene electrodes have good conductivity of similar to 1.1 x 10(3) S cm(-1), flexibility to maintain their electrical connection under static bending, and electrochemical stability in a 15 d accelerated corrosion test. Chronically implanted graphene ECoG devices remain fully functional for up to 180 d, with average in vivo impedances of 24.72 +/- 95.23 k omega at 1 kHz. The ECoG device can measure spontaneous surface field potentials from mice under awake and anesthetized states and sensory stimulus-evoked responses. Significance: The stencil-printing fabrication process can be used to create Graphene ECoG devices with customized electrode layouts within 24 h using commonly available laboratory equipment.